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The growing global population, along with economic considerations, has put the food industry under an increasingly pressing demand for innovative approaches, materials and sources. As a result, the industry has to look into the sources that has been disregarded earlier. In this study, we focused on developing a technological process, which aims to ensure the processing of Baltic sprat (Sprattus sprattus) and Baltic herring (Clupea harengus membras) with minimal losses of valuable raw materials. In this investigati...

The growing global population, along with economic considerations, has put the food industry under an increasingly pressing demand for innovative approaches, materials and sources. As a result, the industry has to look into the sources that has been disregarded earlier. In this study, we focused on developing a technological process, which aims to ensure the processing of Baltic sprat (Sprattus sprattus) and Baltic herring (Clupea harengus membras) with minimal losses of valuable raw materials. In this investigati...

Pollen germination and tube growth of fruit trees are the most important characteristics related to pollen quality. An effective fertilization requires the high rates of germination and fast tube growth. For economic reasons a higher rate of pollen germination is needed in fruit trees. The objective of this study was to investigate the cytological characteristics of pollen grains and analysis the germinative potential and the effect of the different sucrose concentrations on pollen germination and tube growth of s...

Pollen germination and tube growth of fruit trees are the most important characteristics related to pollen quality. An effective fertilization requires the high rates of germination and fast tube growth. For economic reasons a higher rate of pollen germination is needed in fruit trees. The objective of this study was to investigate the cytological characteristics of pollen grains and analysis the germinative potential and the effect of the different sucrose concentrations on pollen germination and tube growth of s...

Studies in the literature on the effect of dietary n-3 and n-6 PUFA on lymphocyte subpopulations in mammalian and poultry studies are contradictory. This might be due to differences in species, basal diet, fat amount, ratio of n-6 to n-3 PUFA and different fatty acids of n-3 PUFA. An inclusion of flaxseed in the diets of chickens can increase the n-3 fatty acids (FA) of poultry meat as it is rich source of ?-linolenic acid. The hepatic lipid metabolism of chickens helps in the incorporation of whole body and muscl...

Studies in the literature on the effect of dietary n-3 and n-6 PUFA on lymphocyte subpopulations in mammalian and poultry studies are contradictory. This might be due to differences in species, basal diet, fat amount, ratio of n-6 to n-3 PUFA and different fatty acids of n-3 PUFA. An inclusion of flaxseed in the diets of chickens can increase the n-3 fatty acids (FA) of poultry meat as it is rich source of ?-linolenic acid. The hepatic lipid metabolism of chickens helps in the incorporation of whole body and muscl...

Cellulose is one of the most important biopolymers and belongs to crystalline polymers. At the same time, it can exist in several crystalline modifications, giving a completely different diffraction pattern, or in an amorphous state. The crystallinity degree of cellulose significantly varies with different chemical and enzymatic treatments. A new method for crystallinity degree determining from X-ray diffraction data has been applied to cellulose samples. A calculation is based on estimating the rate of change in ...

Cellulose is one of the most important biopolymers and belongs to crystalline polymers. At the same time, it can exist in several crystalline modifications, giving a completely different diffraction pattern, or in an amorphous state. The crystallinity degree of cellulose significantly varies with different chemical and enzymatic treatments. A new method for crystallinity degree determining from X-ray diffraction data has been applied to cellulose samples. A calculation is based on estimating the rate of change in ...

Development of food products with high nutritional value is an actual concern for scientist and producers from food industry. Special attention is given to the development of food products for people who have an increased sensitivity to certain foods, like gluten intolerance, which can determine a disease called celiac disease. In this study it has been proposed to obtain a gluten-free product, destined people who suffer from celiac disease but also for children and others. In this study were obtained gluten-free ...

Development of food products with high nutritional value is an actual concern for scientist and producers from food industry. Special attention is given to the development of food products for people who have an increased sensitivity to certain foods, like gluten intolerance, which can determine a disease called celiac disease. In this study it has been proposed to obtain a gluten-free product, destined people who suffer from celiac disease but also for children and others. In this study were obtained gluten-free ...

Wastes from livestock farms contain significant amounts of antibacterial drugs and antibiotic?resistant genes. Due to weak adsorption in the animal's body, antibiotics are released into the environment in an unchanged form or as metabolites in the composition of manure. In addition to the antibacterial agents, the manure of treated animals contains antibiotic?resistant bacteria. The use of manure in untreated form as a soil fertilizer can lead to the spread of antibiotic resistance among soil microorganisms. Compo...

Wastes from livestock farms contain significant amounts of antibacterial drugs and antibiotic?resistant genes. Due to weak adsorption in the animal's body, antibiotics are released into the environment in an unchanged form or as metabolites in the composition of manure. In addition to the antibacterial agents, the manure of treated animals contains antibiotic?resistant bacteria. The use of manure in untreated form as a soil fertilizer can lead to the spread of antibiotic resistance among soil microorganisms. Compo...

Grapevine pruning is one of the most essential tools to ensure grapes quality and efficient yields of vineyards. Vine horticultural performance and leaf physiology of grapevine are influenced by viticultural practices. One of these practices is represented by pruning. The purpose of the present study was to evaluate the effect of pruning system on leaf physiology and vine performance. Currently, viticulture as a sector of agriculture is a priority sector for national and European Union policies that receive suppor...

Grapevine pruning is one of the most essential tools to ensure grapes quality and efficient yields of vineyards. Vine horticultural performance and leaf physiology of grapevine are influenced by viticultural practices. One of these practices is represented by pruning. The purpose of the present study was to evaluate the effect of pruning system on leaf physiology and vine performance. Currently, viticulture as a sector of agriculture is a priority sector for national and European Union policies that receive suppor...

Aflatoxin B1 (AFB1) contamination of coffee beans continues to be a serious issue regarding livestock productivity and human health. AFB1 (secondary metabolites produced by a larger number of Aspergillus spp.) is classified as a Group I carcinogen by the International Agency for Research on Cancer due to the considerable risk to human and animal health. Therefore, monitoring of this toxin by using probiotics during storage became a challenge due to the demand for reduced use of chemical preservatives by consumers....

Aflatoxin B1 (AFB1) contamination of coffee beans continues to be a serious issue regarding livestock productivity and human health. AFB1 (secondary metabolites produced by a larger number of Aspergillus spp.) is classified as a Group I carcinogen by the International Agency for Research on Cancer due to the considerable risk to human and animal health. Therefore, monitoring of this toxin by using probiotics during storage became a challenge due to the demand for reduced use of chemical preservatives by consumers....

Here, we developed an effective D-glucose oxidation with magnetically recoverable biocatalysts. These biocatalysts were synthesized on magnetite particles obtained by different methods, polyol and co-precipitation. These MPs were coated with a layer of silica modified with amino groups. The GOx covalent attachment has been carried out via the reaction with glutaraldehyde. We established that the method of MPs synthesis affect the enzyme catalytic activity. It should be noted that the magnetically recoverable bioca...

Here, we developed an effective D-glucose oxidation with magnetically recoverable biocatalysts. These biocatalysts were synthesized on magnetite particles obtained by different methods, polyol and co-precipitation. These MPs were coated with a layer of silica modified with amino groups. The GOx covalent attachment has been carried out via the reaction with glutaraldehyde. We established that the method of MPs synthesis affect the enzyme catalytic activity. It should be noted that the magnetically recoverable bioca...

Rapeseed, one of the most important crops in the world, gained importance due to the use for food or industrial purposes. For obtaining high yields it is important to consider its requirements related to fertilization. The research was carried out in Ialomita County during the agricultural year 2015-2016, on cambic chernozem using two mid-early rapeseed hybrids: PR44DO6 and Harnas. The objectives of the research consisted in evaluation of the influence of mineral fertilization and hybrid on: rapeseed yield, averag...

Rapeseed, one of the most important crops in the world, gained importance due to the use for food or industrial purposes. For obtaining high yields it is important to consider its requirements related to fertilization. The research was carried out in Ialomita County during the agricultural year 2015-2016, on cambic chernozem using two mid-early rapeseed hybrids: PR44DO6 and Harnas. The objectives of the research consisted in evaluation of the influence of mineral fertilization and hybrid on: rapeseed yield, averag...

The issue of biochar application as a non-conventional fertilizer is of growing interest currently. Biochar is a product of the pyrolysis of organic waste for example and unlike conventional fertilizer, biochar is a long-acting agent. The use of such fertilizer should not only increase the yield of crops, but also the sequestration of carbon in the soil and reduce the impact on climate change. An additional advantage of biochar is its developed surface, which makes it possible to use it as a carrier for beneficial...

The issue of biochar application as a non-conventional fertilizer is of growing interest currently. Biochar is a product of the pyrolysis of organic waste for example and unlike conventional fertilizer, biochar is a long-acting agent. The use of such fertilizer should not only increase the yield of crops, but also the sequestration of carbon in the soil and reduce the impact on climate change. An additional advantage of biochar is its developed surface, which makes it possible to use it as a carrier for beneficial...

Biodiesel is a relatively new type of environmentally friendly fuel, which is made from renewable resources. Biodiesel is a mixture of methyl or ethyl esters of fatty acids. It is obtained by re-esterification of vegetable oils or others fat-containing substrate, including animal origin. Iodine and acid numbers of vegetable oils, such as rapeseed, olive, linseed, nut and sunflower, are determined. In addition, conditions for obtaining biodiesel by en-zymatic catalysis using pancreatic lipase as an enzyme and rapes...

Biodiesel is a relatively new type of environmentally friendly fuel, which is made from renewable resources. Biodiesel is a mixture of methyl or ethyl esters of fatty acids. It is obtained by re-esterification of vegetable oils or others fat-containing substrate, including animal origin. Iodine and acid numbers of vegetable oils, such as rapeseed, olive, linseed, nut and sunflower, are determined. In addition, conditions for obtaining biodiesel by en-zymatic catalysis using pancreatic lipase as an enzyme and rapes...

Whey cheeses, among which Ricotta is the famous one, are considered valuable food products from a nutritional point of view, enabling sustainable valorization of whey, a major by-product of dairy industry. As a consequence of their rich composition, high moisture content and pH, whey cheeses favor microorganism growth. That is why, producers have to pay special attention to avoid spoilage and risks to the consumer?s health, recalls and economic losses. They also need to meet health-conscious consumers demand for h...

Whey cheeses, among which Ricotta is the famous one, are considered valuable food products from a nutritional point of view, enabling sustainable valorization of whey, a major by-product of dairy industry. As a consequence of their rich composition, high moisture content and pH, whey cheeses favor microorganism growth. That is why, producers have to pay special attention to avoid spoilage and risks to the consumer?s health, recalls and economic losses. They also need to meet health-conscious consumers demand for h...

The aim of the research is to characterize chestnut honey by using physicochemical methods to achieve a specific profile. Determination of polyphenols content gives indications of the antioxidant properties of chestnut honey. Changes in quality parameters have been studied regarding acidity, pH, viscosity, dry matter and anthocyanin content. The obtained results indicate variations in the pH of honey samples between 4.9-6.7, the acidity of honey samples varied from 0.21 to 0.41. The honey viscosity was determined ...

The aim of the research is to characterize chestnut honey by using physicochemical methods to achieve a specific profile. Determination of polyphenols content gives indications of the antioxidant properties of chestnut honey. Changes in quality parameters have been studied regarding acidity, pH, viscosity, dry matter and anthocyanin content. The obtained results indicate variations in the pH of honey samples between 4.9-6.7, the acidity of honey samples varied from 0.21 to 0.41. The honey viscosity was determined ...

Research aim of the present paper is to present an evaluation of the presence on wild strawberry (Fragaria vesca) of one of the most important leaf pathogens which create yield loses to common cultivated strawberry cultivars from the western part of Romania. This pathogen is Mycosphaerella fragariae. The biologic material consist from an assortment of four different population of wild strawberries. Technology used in the experimental field was the standard applied for this location. The experience was implemented ...

Research aim of the present paper is to present an evaluation of the presence on wild strawberry (Fragaria vesca) of one of the most important leaf pathogens which create yield loses to common cultivated strawberry cultivars from the western part of Romania. This pathogen is Mycosphaerella fragariae. The biologic material consist from an assortment of four different population of wild strawberries. Technology used in the experimental field was the standard applied for this location. The experience was implemented ...

Sorghum (Sorghum bicolor L.) is among the top five most cultivated and most important cereals in the world, being a highly yielded crop that adapts easily to drought and high temperatures. Besides being used as a raw material for biofuel, it is used in the diet in many parts of the world because of the rich content of health promoting compounds such as phenolic compounds. The aim of this research was to determine the total phenolic content, flavonoids content and the antioxidant activity of flour made from whole g...

Sorghum (Sorghum bicolor L.) is among the top five most cultivated and most important cereals in the world, being a highly yielded crop that adapts easily to drought and high temperatures. Besides being used as a raw material for biofuel, it is used in the diet in many parts of the world because of the rich content of health promoting compounds such as phenolic compounds. The aim of this research was to determine the total phenolic content, flavonoids content and the antioxidant activity of flour made from whole g...

The study presents the comparative analysis between pressure and carbon dioxide charts obtained after measuring a series of fermentation processes. Doughs used for measurements were obtained from different quality/extraction flours, type 650 and whole wheat (1850). Gathered data was used to verify a mathematical model for determination of carbon dioxide production during the fermentation process, using pressure variation in a sealed container (reactor). For the testing it was used an experimental stand composed of...

The study presents the comparative analysis between pressure and carbon dioxide charts obtained after measuring a series of fermentation processes. Doughs used for measurements were obtained from different quality/extraction flours, type 650 and whole wheat (1850). Gathered data was used to verify a mathematical model for determination of carbon dioxide production during the fermentation process, using pressure variation in a sealed container (reactor). For the testing it was used an experimental stand composed of...

The aim of the study was to compare the functional and technological properties of protein concentrates (PC), protein-calcium composites (PCC) and protein concentrates hydrolyzed by endoprotease and exopeptidase (HPC) with a degree of proteolysis of 3.89-4.16%. All kinds of concentrates were obtained from white and brown rice. It was found that the functional properties are higher in BC obtained from white rice than in brown rice rice. Thus, the foaming capacity is 5-6 times higher, but the stability of foam in br...

The aim of the study was to compare the functional and technological properties of protein concentrates (PC), protein-calcium composites (PCC) and protein concentrates hydrolyzed by endoprotease and exopeptidase (HPC) with a degree of proteolysis of 3.89-4.16%. All kinds of concentrates were obtained from white and brown rice. It was found that the functional properties are higher in BC obtained from white rice than in brown rice rice. Thus, the foaming capacity is 5-6 times higher, but the stability of foam in br...

Waste products of plant origin are valuable secondary raw materials in the process of agricultural wastes utilization into products with high added value. The application of secondary raw materials is complicated due to the high fibrecontent. The aim of this study was to identify strains of microorganisms capable to produce a wide range of exocellulolases in order to reduce the crude fibrein mild conditions without applying expensive commercial cellulases. The following strains were chosen for this experiment: Tri...

Waste products of plant origin are valuable secondary raw materials in the process of agricultural wastes utilization into products with high added value. The application of secondary raw materials is complicated due to the high fibrecontent. The aim of this study was to identify strains of microorganisms capable to produce a wide range of exocellulolases in order to reduce the crude fibrein mild conditions without applying expensive commercial cellulases. The following strains were chosen for this experiment: Tri...

The fast development in chromatography occurred in the middle of twentieth century when gas chromatography came to light. It was invented by James and Martin. Presently, gas chromatography coupled with universal or selective detectors including mass selective detector is an effective, powerful and versatile analytical tool for special cases. It can be applied in numerous fields for example in drugs and pharmaceuticals, clinical chemistry, foods, environmental analysis or petroleum industry. Gas chromatography can ...

The fast development in chromatography occurred in the middle of twentieth century when gas chromatography came to light. It was invented by James and Martin. Presently, gas chromatography coupled with universal or selective detectors including mass selective detector is an effective, powerful and versatile analytical tool for special cases. It can be applied in numerous fields for example in drugs and pharmaceuticals, clinical chemistry, foods, environmental analysis or petroleum industry. Gas chromatography can ...

The study of halobacterial communities is a promising direction in applied and theoretical biotechnology. Researches, carried out in this field, open up new approaches in understanding the role of interspecies interactions between halobacterial cells in conditions of increased salinity. Viruses, as one of the major regulation factor, greatly affect the composition of communities. Every year the halovirus database is replenished with new representatives, but still much remains unknown about the life cycle of viruse...

The study of halobacterial communities is a promising direction in applied and theoretical biotechnology. Researches, carried out in this field, open up new approaches in understanding the role of interspecies interactions between halobacterial cells in conditions of increased salinity. Viruses, as one of the major regulation factor, greatly affect the composition of communities. Every year the halovirus database is replenished with new representatives, but still much remains unknown about the life cycle of viruse...

The stoichiometry of metal?protein complexes is pH dependent. Since mass spectrometric measurements at higher pH values are difficult in the presence of usual buffers, we extended this approach to pH in excess of 10 by adding ethanolamine to the electrospray solution being investigated. Therefore, a new method for probing the metal ion-peptide complexes at high pH utilizing electrospray ionization mass spectrometry (ESI-MS) has been advanced. Nevertheless, the high pH ESI-MS spectra of peptides are far too complex...

The stoichiometry of metal?protein complexes is pH dependent. Since mass spectrometric measurements at higher pH values are difficult in the presence of usual buffers, we extended this approach to pH in excess of 10 by adding ethanolamine to the electrospray solution being investigated. Therefore, a new method for probing the metal ion-peptide complexes at high pH utilizing electrospray ionization mass spectrometry (ESI-MS) has been advanced. Nevertheless, the high pH ESI-MS spectra of peptides are far too complex...

Alzheimer?s disease (AD) is associated with conformational changes of amyloid-? peptides, which result in peptide oligomerization and fibril formation and, finally, in the appearance of senile plaques and extensive neuronal loss. However, the factors regulating the process of plaque-associated beta-amyloid peptide (A?) aggregation are only partly understood. Therefore, several ?-amyloid peptide fragments have been synthesized by SPPS according to Boc/Bzl strategy. Binding of metal ions to ?-amyloid peptides was st...

Alzheimer?s disease (AD) is associated with conformational changes of amyloid-? peptides, which result in peptide oligomerization and fibril formation and, finally, in the appearance of senile plaques and extensive neuronal loss. However, the factors regulating the process of plaque-associated beta-amyloid peptide (A?) aggregation are only partly understood. Therefore, several ?-amyloid peptide fragments have been synthesized by SPPS according to Boc/Bzl strategy. Binding of metal ions to ?-amyloid peptides was st...

Molluscs are invertebrates that form one of the largest groups in the animal kingdom, with more than 100,000 known species. A wide range of molluscs including families of Pulmonata, Gastropoda, Planorbidae and Lymnaeidae are intermediate hosts of trematodes. The snail Lymnaea stagnalis belongs to the family Lymnaeidae under the order Basommatophora-class Pulmonata is known to be an intermediate host of several parasites such as Chaetogaster limnaei which belongs to the annelid family, Naididae, an ecologically div...

Molluscs are invertebrates that form one of the largest groups in the animal kingdom, with more than 100,000 known species. A wide range of molluscs including families of Pulmonata, Gastropoda, Planorbidae and Lymnaeidae are intermediate hosts of trematodes. The snail Lymnaea stagnalis belongs to the family Lymnaeidae under the order Basommatophora-class Pulmonata is known to be an intermediate host of several parasites such as Chaetogaster limnaei which belongs to the annelid family, Naididae, an ecologically div...

The magnetically recoverable biocatalyst was developed. The catalyst support is based on magnetite particles (MPs) composed of magnetite particles and coated with the amino terminated silica layer to facilitate further functionalization. This functionalization involves the attachment of the glutaraldehyde linker followed by the covalent attachment of horseradish peroxidase (HRP) via its amino groups. The catalyst was tested in the reaction of 2,3,6-trimethylphenol (TMP) oxidation to 2,3,5-trimethylhydrochinone (TM...

The magnetically recoverable biocatalyst was developed. The catalyst support is based on magnetite particles (MPs) composed of magnetite particles and coated with the amino terminated silica layer to facilitate further functionalization. This functionalization involves the attachment of the glutaraldehyde linker followed by the covalent attachment of horseradish peroxidase (HRP) via its amino groups. The catalyst was tested in the reaction of 2,3,6-trimethylphenol (TMP) oxidation to 2,3,5-trimethylhydrochinone (TM...