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Zein proteins belong to the class of prolamin proteins which, in maize, represent more than 60% of the total endosperm protein. Based on their molecular weight, sequence and solubility, zeins are classified into four different zein structures (?, ?, ?, ?). The major maize seed storage proteins, zeins, are deficient in lysine and tryptophan content, which contribute to the poor nutritional quality of corn. Therefore, we report here on an original and novel process, which consists in successive grinding and sieving ...

The purpose of the research is to develop multifunctional biological products based on the utilization of organic waste and raw materials from components of animal and plant origin; to study their impact on the restoration of soil fertility and productivity of field and garden crops. As a result, the assortment line of biological products of the Agrotonic trademark Was developed: "flower Grower", "Gardener", "vegetable Grower", "field Grower" and "forest Grower". Each type of "AG-rotonika" is also divided into sub...

Alzheimer?s disease, the most common age-related dementia worldwide, is characterized by two pathologies: deposition of ?-amyloid plaque and neurofibrillary tangles of hyperphosphorylated tau. To this date, several factors, such as oxidative stress, metal ions, or genetic factors are associated with Alzheimer`s diseases, but there are still some question marks regarding: (i) how the misfolding and aggregation of A? occurs and which is the aggregation mechanism; (ii) how the metal ions binds to A? and how they init...

Modern industrial biotechnology considers extremophilic microorganisms and, in particular, extreme halophiles as promising producers for the bioeconomy of the future. Extremely halophilic bacteria and archaea can be used for the production of carotenoids, polyhydroxyalkanoates, polysaccharides, osmoprotectors, thermostable enzymes, photosystem proteins, and can also be sources of unique biologically active substances for medical purposes. The implementation of large-scale industrial processes with minimal requirem...

To date, there is an increase in the incidence of skin and subcutaneous tissue diseases, despite the variety of treatment methods. One of the promising class of biomaterials for the treatment of skin diseases can be coatings based on hydrogels. The purpose of this study was to obtain collagen-acrylic hydrogels with various technological parameters (varying the synthesis temperature and the degree of neutralization of acrylic acid) with the specified properties by radical polymerization. The chemical structure of t...

This study is devoted to studying the properties of a new polymer sorbent based on polyvinyl alcohol and carboxymethylcellulose using infrared spectroscopy and thermogravimetric analysis. The influence of calcium chloride on the performance characteristics of the sorbent, such as oil capacity, floatation, moisture content and polymer degradation, is studied. The cross-linked matrix has improved properties: increasing the oil capacity and flotation time, retention of the structure when the sorbent is on the water s...

This work is focused on antioxidant activity of various types of horticultural crops. Several types of vegetable and fruits were analyzed by free radicals scavenging method using 1,1-diphenyl-2-picrylhydrazyl (DPPH). In this work various methods of analysis results expressing were evaluated. The results were expressed, in terms of the widest applicability, while the simplicity of the serial analysis were maintained. Percentage of inhibition of free radicals was expressed per unit sample weight and standard pipetti...

Bacteriorhodopsin (bR) is a red-colored protein found in cell membranes of the organism Halobacterium salinarum, being considered as a light activated proton pump that transports protons across the plasma membrane. Because bacteriorhodopsin is one of the simplest ATP-producing systems known in biology, it has been the subject of intensive investigations. The bR photo-intermediates were seen in the mechanistic model considering that this protein links light energy to the translocation of protons across the bacteria...

A research of edible gelatin rainbow holograms is presented. Gelatin is an edible material with optical properties: it is transparent in the visible region of the spectrum and has refractive index close to refractive index of optical polymers. Gelatin is capable to transfer diffraction relief and save it for a long time. Gelatin holograms were prepared by nanoimprinting method from gelatin aqua solutions with different concentrations (1 part of gelatin per 5, 10, 15, 20, 25 parts of water). The samples were made o...

Amiodarone (AMD) is an antiarrhythmic agent included in the 2nd class according to the biopharmaceutical classification of drug substances. The oral bioavailability of AMD in conventional pharmaceutical formulations exhibits large interindividual variations due to the rapid dealkylation of the molecule to desethylaminodarone (inactive metabolite) and due to low hydrosolubility. The complexation of the drug substance in the central cavity of various kinds of cyclodextrins is one of the most effective methods for op...

Model for a change of the biosystems states on the grounds of Markov chains is offered in the work. One of the main problems of management of the biosystems states is the study of the mechanisms, which determine dynamic features of the processes, taking place in the natural environment. Therefore, there is a necessity in the development of special methods, allowing to study dynamic properties of natural objects without subjecting them to ruination. Naturally, a mathematical modeling method may satisfy this demand ...

Frozen fish is the primary raw material used in the industrial preparation of semi-finished ready to eat fish-based products. Ready to eat fish product manufacturers receive frozen fish raw materials from suppliers and defrost it before processing. Such raw materials are commodities; frozen fish are easily transported around the world, can be stored for a long time, and used for further processing as required, evenly distributing the company?s production capacity throughout the year and, thus, optimising costs. No...

A vital role in detecting eye disorders is played by the analysis of the human retina using medical imaging methods. The main objective of this study is to improve the detection of retinal blood vessels by ameliorating the overall quality of non-uniform illumination conditions, removing image artifacts, connecting the broken vessels, and generating accurate segmented retinal vascular systems. A three-stage edge detection framework is proposed. First, image denoising, contrast enhancement, stretched decorrelation a...

Bacillus megaterium is a bacterium that has been used for the industrial production of vitamin B12 (cobalamin), the anti-pernicious anaemia factor. Cobalamin is a modified tetrapyrrole with a cobalt ion coordinated within its macrocycle. Previous findings outline a potential way to develop B. megaterium as a cell factory for cobalamin production using cheap raw materials. In this paper, a novel strategy was implemented to investigate the possibility to use sunflower protein isolates and its enzymatic hydrolysates ...

For to determinate the DNA content and analysis of cell distribution in different phases of the cell cycle, flow cytometry was used. The exact measurements of a large number of nuclei by flow cytometry, fluorescently labeled by passing a laser beam (excitation), which marks each individual nucleus by fluorescence, allows a rapid estimation of the relative nuclear content of the DNA of the analyzed plants . Approximately 0.5 cm2 of foliar tissue is harvested, crushed very well, followed by incubation of the sample ...

For to determinate the DNA content and analysis of cell distribution in different phases of the cell cycle, flow cytometry was used. The exact measurements of a large number of nuclei by flow cytometry, fluorescently labeled by passing a laser beam (excitation), which marks each individual nucleus by fluorescence, allows a rapid estimation of the relative nuclear content of the DNA of the analyzed plants . Approximately 0.5 cm2 of foliar tissue is harvested, crushed very well, followed by incubation of the sample ...

The research carried out in the present paper aimed at monitoring and evaluating the technological process of obtaining the ecological cheese type ?PETROVA?, in the Maramures county area, Romania. To achieve this raw milk originating from domestic producers was evaluated in terms of fat content, acidity and density of this. After the final ecological cheese type product has been obtained, analyzes have been made for it?s physicochemical evaluation as follows: water content analysis, fat content analysis, salt cont...

The research carried out in the present paper aimed at monitoring and evaluating the technological process of obtaining the ecological cheese type ?PETROVA?, in the Maramures county area, Romania. To achieve this raw milk originating from domestic producers was evaluated in terms of fat content, acidity and density of this. After the final ecological cheese type product has been obtained, analyzes have been made for it?s physicochemical evaluation as follows: water content analysis, fat content analysis, salt cont...

The research carried out in this paper aimed to evaluate five types of beer throughout the technological flow of their production. The technological flow of producing the five types of beer has been monitored at all stages of it, starting with beer must, beer before filtering, BBT beer and bottled beer. To achieve this goal, the following physicochemical analyzes were carried out: color analysis expressed in EBC units, pH analysis, determination of bitter substances expressed in units of B.U. All the results obtai...

The research carried out in this paper aimed to evaluate five types of beer throughout the technological flow of their production. The technological flow of producing the five types of beer has been monitored at all stages of it, starting with beer must, beer before filtering, BBT beer and bottled beer. To achieve this goal, the following physicochemical analyzes were carried out: color analysis expressed in EBC units, pH analysis, determination of bitter substances expressed in units of B.U. All the results obtai...

An actual problem of the bread making industry is represented by the final properties of the desired product. This fact requires a well knowledge of the technological process, the properties of the flour used, the proportions and the dough mixing method, factors that contribute to the desirable properties of the final products. This study aims at comparing the two basic methods of dough obtaining ? the direct method and the indirect method, with direct reference to the dough improved with lentil flour in addition ...

An actual problem of the bread making industry is represented by the final properties of the desired product. This fact requires a well knowledge of the technological process, the properties of the flour used, the proportions and the dough mixing method, factors that contribute to the desirable properties of the final products. This study aims at comparing the two basic methods of dough obtaining ? the direct method and the indirect method, with direct reference to the dough improved with lentil flour in addition ...

The growing global population, along with economic considerations, has put the food industry under an increasingly pressing demand for innovative approaches, materials and sources. As a result, the industry has to look into the sources that has been disregarded earlier. In this study, we focused on developing a technological process, which aims to ensure the processing of Baltic sprat (Sprattus sprattus) and Baltic herring (Clupea harengus membras) with minimal losses of valuable raw materials. In this investigati...

The growing global population, along with economic considerations, has put the food industry under an increasingly pressing demand for innovative approaches, materials and sources. As a result, the industry has to look into the sources that has been disregarded earlier. In this study, we focused on developing a technological process, which aims to ensure the processing of Baltic sprat (Sprattus sprattus) and Baltic herring (Clupea harengus membras) with minimal losses of valuable raw materials. In this investigati...

The study presents the comparative analysis between pressure and carbon dioxide charts obtained after measuring a series of fermentation processes. Doughs used for measurements were obtained from different quality/extraction flours, type 650 and whole wheat (1850). Gathered data was used to verify a mathematical model for determination of carbon dioxide production during the fermentation process, using pressure variation in a sealed container (reactor). For the testing it was used an experimental stand composed of...

The study presents the comparative analysis between pressure and carbon dioxide charts obtained after measuring a series of fermentation processes. Doughs used for measurements were obtained from different quality/extraction flours, type 650 and whole wheat (1850). Gathered data was used to verify a mathematical model for determination of carbon dioxide production during the fermentation process, using pressure variation in a sealed container (reactor). For the testing it was used an experimental stand composed of...

The stoichiometry of metal?protein complexes is pH dependent. Since mass spectrometric measurements at higher pH values are difficult in the presence of usual buffers, we extended this approach to pH in excess of 10 by adding ethanolamine to the electrospray solution being investigated. Therefore, a new method for probing the metal ion-peptide complexes at high pH utilizing electrospray ionization mass spectrometry (ESI-MS) has been advanced. Nevertheless, the high pH ESI-MS spectra of peptides are far too complex...

The stoichiometry of metal?protein complexes is pH dependent. Since mass spectrometric measurements at higher pH values are difficult in the presence of usual buffers, we extended this approach to pH in excess of 10 by adding ethanolamine to the electrospray solution being investigated. Therefore, a new method for probing the metal ion-peptide complexes at high pH utilizing electrospray ionization mass spectrometry (ESI-MS) has been advanced. Nevertheless, the high pH ESI-MS spectra of peptides are far too complex...

Winter pumpkin represents a seasonal crop of health and food interest due to its nutritional composition. The aim of the present study was to comparatively evaluate the content of the main antioxidant compounds, carotenoids and phenolics, from pumpkin (Cucurbita maxima) pulp as related to different pretreatment conditions. Samples of pumpkin pulp were subjected to three pre-extraction methods, as follow: freeze-drying, oven-drying at 60oC and freezing at -70oC. Conventional extraction of carotenoids and polyphenol...

Winter pumpkin represents a seasonal crop of health and food interest due to its nutritional composition. The aim of the present study was to comparatively evaluate the content of the main antioxidant compounds, carotenoids and phenolics, from pumpkin (Cucurbita maxima) pulp as related to different pretreatment conditions. Samples of pumpkin pulp were subjected to three pre-extraction methods, as follow: freeze-drying, oven-drying at 60oC and freezing at -70oC. Conventional extraction of carotenoids and polyphenol...

The use of various ingredients in the formulation of yogurt, in order to diversify a dairy product particularly appreciated by consumers, is a constant concern for industry specialists. The paper proposes the use pumpkin seeds in the recipe of a classic yogurt, in order to improve its physico-chemical, textural and sensory characteristics. Pumpkin seeds, like other oilseeds, are in the attention of specialists because they are a very good source of proteins and dietary fibers. For the consumer, the increase in the...

The use of various ingredients in the formulation of yogurt, in order to diversify a dairy product particularly appreciated by consumers, is a constant concern for industry specialists. The paper proposes the use pumpkin seeds in the recipe of a classic yogurt, in order to improve its physico-chemical, textural and sensory characteristics. Pumpkin seeds, like other oilseeds, are in the attention of specialists because they are a very good source of proteins and dietary fibers. For the consumer, the increase in the...

In this paper we used the research done in a previous laboratory study in which we optimized the addition of seabuckthorn powder, rosehip powder and grape seed extract to the classic yogurt formulation. Yogurt with the best physicochemical and sensory properties was obtained by adding 1.5% rosehip powder and 0.5% grape seed extract, respectively 1.75% seabuckthorn powder and 0.25% grape seed extract to its manufacturing formulation. Prior to inoculation, the ingredients mixture was pasteurized at 85В°C for 30 minu...

In this paper we used the research done in a previous laboratory study in which we optimized the addition of seabuckthorn powder, rosehip powder and grape seed extract to the classic yogurt formulation. Yogurt with the best physicochemical and sensory properties was obtained by adding 1.5% rosehip powder and 0.5% grape seed extract, respectively 1.75% seabuckthorn powder and 0.25% grape seed extract to its manufacturing formulation. Prior to inoculation, the ingredients mixture was pasteurized at 85В°C for 30 minu...

The paper presents the impact of variable environment acidity and low temperature on the stability of biosensor receptor layer component BSA (Bovine Serum Albumin). With the use of UV/Vis spectrophotometer, absorption spectra in range (250-300nm) was taken to study conformational changes of the protein with time after exposition to different factors. The results showed an increase in absorption, associated with the conformational changes of BSA with time. A significant decrease in the absorption intensity was note...

The paper presents the impact of variable environment acidity and low temperature on the stability of biosensor receptor layer component BSA (Bovine Serum Albumin). With the use of UV/Vis spectrophotometer, absorption spectra in range (250-300nm) was taken to study conformational changes of the protein with time after exposition to different factors. The results showed an increase in absorption, associated with the conformational changes of BSA with time. A significant decrease in the absorption intensity was note...

Development and consumption of frozen products is an aspect that is strongly related to nowadays consumers. These are considered less nutritious and less healthy than fresh ones due to processing, artificial ingredients and additives used for production. The paper focuses on the influence of frozen storage of bread dough enriched with lentil flour. The addition of lentil flour in different proportions (15% and 30%) was investigated in order to study the physicochemical and organoleptic effect concerning the obtain...

Development and consumption of frozen products is an aspect that is strongly related to nowadays consumers. These are considered less nutritious and less healthy than fresh ones due to processing, artificial ingredients and additives used for production. The paper focuses on the influence of frozen storage of bread dough enriched with lentil flour. The addition of lentil flour in different proportions (15% and 30%) was investigated in order to study the physicochemical and organoleptic effect concerning the obtain...

The bioconversion of plant raw materials into highly added value products, as well as the development of new products with advanced characteristics has a high priority for modern biotechnology in the Russian Federation. Deproteinized sunflower meal has been used as a source of carbohydrates and organic nitrogen for the yeast S. cerevisiae fermentation after subsequent acidic and enzymatic treatment. As a result a protein-rich yeast biomass was formed, as well as a liquid fraction, containing non-metabolized pentos...

The bioconversion of plant raw materials into highly added value products, as well as the development of new products with advanced characteristics has a high priority for modern biotechnology in the Russian Federation. Deproteinized sunflower meal has been used as a source of carbohydrates and organic nitrogen for the yeast S. cerevisiae fermentation after subsequent acidic and enzymatic treatment. As a result a protein-rich yeast biomass was formed, as well as a liquid fraction, containing non-metabolized pentos...

The purpose of this study is to highlight the flavour compounds found in tonic beverages obtained from plants with bioactive potential. Identifying these compounds through GC-FID (gas-chromatographic coupled with flame ionization detector) modern methods are leading to the appreciation of these beverages and the possibility of improving the proposed recipes. Six drinks rated B1, B2, B3, B4, B5, B6 made from indigenous plants; the plants were dried at 65 пїЅ C, macerated in 50% vol. ethanol for three days in a rati...

On the last four years, we take surveys on the diseases of some plants from natural habitats which, over time, support improvement by plant breeding process One of the locations where we can found such natural resources is the Nera River valley, inside of the National Park, and those is the main reason we are sure that this plants are on their natural habitat. Regarding elderberries shrubs (Sambucus nigra) on the research area, they are well represented as populations along the river sides. It is well known the ut...

Ocimum basilicum L. (Lamiaceae) is a spice plant frequently cultivated in Romania with application in food flavoring and preservation, pharmaceutical products and alternative medicine. The purpose of this study was to determine the chemical composition and antioxidant effectiveness of the essential oil of Ocimum basilicum grown in Romania. The basil inflorescences (fresh plant material) was used for the extraction of essential oil. The essential oil was isolated with a yield of 0.52% (v/m) by steam distillation. G...

In recent years, the ultrasonic field has gained the attention of several authors in the area of employing it to process and interact with fruit juices as a viable alternative to the use of conventional processing technologies. The ultrasound technique main advantage, as a non-thermal treatment, is its ability to maintain juice quality whist enhancing the juice homogenization level. The aim of this study was to subject the sea buckthorn juice to ultrasonic field treatment in order to improve the its pulp stability...

Novel trends in the market of fermented dairy products are leading to obtain various products with high functional and nutritive but less energy value. The development of new fermented dairy products could be divided into two groups in order to: 1. obtain products with improved physicochemical characteristics, rheology, texture, microstructure and sensory properties; 2. obtain products with pronounced nutritive characteristics and functionality. Yogurt is a very popular dairy product in most countries, it is consi...

The reactive oxygen species are usually generated by normal cellular activities of the aerobic metabolism and play the role of a signal molecule and beneficial growth regulator. When plants are exposed to stress conditions the concentration of reactive oxygen species increases. Therefore, to neutralize these free radicals and to protect cells from oxidative damage enzymatic and non-enzymatic antioxidant systems are activated. In this paper it was investigated the effect of drought on the activities of antioxidant ...

Natural compounds and products are increasingly demanded by the pharmaceutical and cosmetics industries as safer and user friendly alternatives to synthetic variants. Young wheat plantlets, known as wheatgrass, are a valuable source of minerals, vitamins and chlorophyll pigments and the biosynthesis of such compounds may be influenced by the cultivation conditions. The present study focuses onto the chemical profile, mineral and chlorophyll content of cultivated wheatgrass using two different types of water (sprin...

The aim of this study was to analyzed the effect of calcium ions from gluconate and lactate salt at the level of 100, 150 and 200 mg/100g addition on empirical dough rheological properties from the wheat flour type 1250. Dough empirical rheological properties were analyzed using the Farinograph, Amilograph, Glutograph, Alveograph, and Rheofermentometer devices. These devices allow us to obtain complete informations about dough rheological properties during mixing, pasting and fermentation process of wheat flour do...

The purpose of this research was to improve the nutritional and sensory properties of butter biscuits by addition of grape pomace. The pomace derived from two grape varieties, Pinot Noir and Italian Riesling, was dried at 60пїЅC for 8 hours daily, three days in a row. In the butter biscuits recipe, the wheat flower was replaced with three doses of grape pomace (10, 20 and 30%) added in the form of powder. A control sample, without any addition of grape pomace, was also prepared. The biscuits were analyzed in terms...

The purpose of this study was to determine an optimal variant for producing skimmed stirred yoghurt with chia powder. Three variants of product with chia powder at levels of 0.3%, 0.5%, respectively 0.7% and two control samples have been manufactured. Chia was used due to its remarkable content in fibre, ?-3 and ?-6 fatty acids, polyphenols, which are essential compounds for a healthy human body. The physicalchemical parameters (acidity, pH, dry matter) and rheological properties of yogurts samples were determined...