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Annually, a city with 500,000 inhabitants generates approximately 115.5 million cubic meters of wastewater. The treatment of this wastewater is efficiently managed using activated sludge, which comprises a diverse community of protozoa, infusoria, lower crustaceans, algae, and microorganisms. These organisms facilitate the effective treatment of large volumes of wastewater in treatment plants. However, the activated sludge proliferates by consuming organic materials in the wastewater, resulting in the accumulation...

Biochar is a highly porous pyrolysis product with a high specific surface area and adsorption properties, capable of adsorbing various compounds and formations, including ARG-containing MGEs. To assess the ability of biochar to adsorb MGE, a model experiment was carried out: in sterill flasks, the microbial community isolated from composts was cultivated on LB-medium, oxytetracycline and a mixture of heavy metals were added as factors potentially stimulating the production of MGE. Neither heavy metals nor oxytetra...

Soil contamination by polycyclic aromatic hydrocarbons (PAHs) is a widespread concern due to the high potential for human exposure through inhalation, ingestion, and dermal contact. In this study, we compared the efficiency of three different methods for PAHs extraction from model samples, including ultrasonic (US), mechanical shaking and purification with commercial QuEChERS kit (Shq), and ultrasonic extraction coupled with QuEChERS (USq) by gas chromatography coupled to a triple quadrupole tandem mass-spectromet...

Surfactants play an important role in enhanced oil recovery by creating an oil-water emulsion that will be easier to recover from the reservoir. It is known that sulfatereducing bacteria (SRB) can use surfactants as a carbon source, which can increase the biological accumulation of sulfides and, accordingly, the corrosion of oil production equipment. Thus, all new surfactants used in oil production need to be pre-tested for SRB. The aim of this study was to evaluate the effect of four new groups of synthesized sur...

The use of microalgae as carbon sequestrators requires optimization of the cultivation process. Growth curves for three types of microalgae were plotted. The growth was carried out at different temperatures 30 and 35 ? and with initially different concentrations of inoculum 30 and 3% in order to assess the possibility of growing crops at the lowest cost and with a high yield of a useful product. We used 3 strains of microalgae - potential sequestrators. The introduction of the inoculum at a dose of 30% led to an a...

Suppression of fungal phytopathogens is one of the serious challenges agriculture and forestry since they cause significant biomass and yield losses. One of the alternative methods of suppression phytopathogens is the use of biosurfactants, which are amphiphilic substances produced by many microbes. Bacteria of Pseudomonas genus are known to produce biosurfactants of the rhamnolipid class. These surfactants can possess antimicrobial and fungicidal properties. In this work, we studied the effect of various concentr...

Many oilfields such as supergiant Romaschkinskoye oilfield in Russia contain heavy oils that are complicated to recover. In order to increase the proportion of obtained oil, methods of enhanced oil recovery are implemented, and microbial methods (MEOR) are considered as safe and efficient once. In-situ MEOR are based on stimulation of oilwell microflora that partly decomposes hydrocarbon molecules or alters oil-water interface tension. Despite oilwells are an environments with anaerobic conditions, many allochtono...

Microbial communities associated with the plant rhizosphere play an important role in carbon sequestration, regulation of nutrient cycling, and the efficient functioning of the ecosystem as a whole. The diversity of microorganisms inhabiting the plant rhizosphere and their complex interactions with the host plant significantly affect the morphology, physiology, growth, development, and health of plants. At the same time, it is known that the soil microbiome diversity is affected by the type of soil, the type of cu...

Today there is a serious environmental problem - global climate change, and one of the main reasons for this change is the release of so-called greenhouse gases, and primarily raw carbon dioxide. One strategy to reduce the concentration of carbon dioxide in the atmosphere is to capture carbon dioxide from industrial emissions, since this is where the gas is found in high concentrations. In addition to physical and chemical methods, carbon dioxide capture is possible with the help of biological methods, for example...

Chicken manure is a source of biogenic elements and therefore can be used as a nonconventional fertilizer after composting. However, chicken manure can contain different hazardous substances including antibiotic resistant bacteria and corresponding genes of antibiotic resistance that can be transmitted to human and livestock trough soils and plants even after composting. Besides chicken manure composts can contain heavy metals that not only harm soils and yields directly but also stimulate the process of antibioti...

Pollution of aquatic ecosystems with antibiotic-resistant genes originating from human medicine and veterinary is an urgent problem due to the potential threaten to public health. Antibiotic-resistant genes enter surface waters and wastewater through vertical and horizontal water runoff. At the same time, heavy metals and biogenic substances often presented in aqueous ecosystems often exacerbate the problem since the drive the horizontal transfer of antibiotic-resistance genes. To solve the problem of purification...

Global climate change is one of the most acute environmental problems of our time. Among the methods of capturing CO2 in industrial emissions are chemical, physical and biological. The latter are based on the use of living organisms that absorb carbon dioxide in metabolic processes, and the subsequent use or burial of their biomass or their metabolic products. One variant of organisms used to capture carbon dioxide biotechnologically are microalgae. In the present study, the efficiency to capture carbon from the e...

The anthropogenic influence on petroleum reservoirs during oil extraction leads to the emergence of new electron acceptors and donors and exogenous microorganisms. Microbial biodegradation of hydrocarbons in petroleum reservoirs leads to a change of its composition, resulting in a decrease in its cost. The identification of microorganisms capable of in-situ degradation of hydrocarbons under reservoir conditions is critical to understand the role of microbes in hydrocarbon conversion. To analyze microbial biodegrad...

Public gardens and park zones are an important and widely visited component of urban spaces worldwide, however they can be polluted and even dangerous for humans and animals visiting them. The simplest way to screen the level of pollution of those green zones is to assess their ecological toxicity. In the present study, to assess the ecotoxicity of soil and ground samples obtained in 15 parks and public gardens in the city of Kazan (Russia) with 1 Mio citizens were investigated. Two elutriate assays with Ceriodaph...

The problem of carbon dioxide emission from the soil is relevant, since it affects the reduction of the soil organic carbon pool, thereby reducing its fertility and productivity. In addition, carbon dioxide emissions from the soil are a significant contributor to the greenhouse effect. The aim of this work was to evaluate the mineralizing activity of the microbial community of the soil of a new point of observation of carbon dioxide emissions, located in the previously little-studied territory of Russia, using suc...

The present work is an attempt to create a biosurfactant producing consortia on the bases of initial rhizospheric community of lettuce plant (Lactuca sativa). To obtain consortia, 47 strains from the rhizosphere were isolated and checked upon their ability to produce biosurfactants. The ability of the isolates to produce biosurfactants was analyzed on the basis of their ability to emulsify crude oil (E24 index). The isolates with the highest E24 were Bacillus. oryzaecorticis (80%), B. simplex (65%), Paenibacillus ...

Chicken manure is one of the most wide spread waste worldwide. One of its hazardous properties is contamination with live pathogens or pathogens- spores. Being introduced into soil for fertilization, fresh, cured or treated manure can cause soil contamination with those pathogens. Further, transmission of the pathogens through soil and plant tissues to human or animal food is possible. The objective of the present work was to reveal the level of pathogenic contamination of wheat grains cultivated on soil that was ...

The number of registered cases of COVID-19 is increasing in the world, and some countries are reporting a second wave of the pandemic. Accurate and real time information about epidemiological situation is therefore urgently needed for managing decisions in the countries, regions and municipalities which are affected. Massive testing of viral presence in people’s saliva, a smear from the nose, nasopharynx and / or oropharynx, bronchial lavage water obtained by fibrobronchoscopy (bronchoalveolar lavage), as well as ...

It is widely known that oil production processes do not usually achieve maximum efficiency. One method of increasing production is microbial enhanced oil recovery (MEOR). MEOR methods can clean oil, increase production and clean oil-contaminated soils by not only introducing the microorganisms themselves but also the substances they produce. Among the advantages of these methods are their relative efficiency and the absence of negative impact on the environment. MEOR methods are divided into two groups the first g...

The problem of the antibiotic resistance of bacteria inhabiting the guts of poultry and livestock and furthermore manures and fertilizers prepared from manures is recognized as one of the most serious problems in agriculture. Composting is described as an effective way to reduce the abundance of antibiotic-resistant genes in the final product, however such a positive effect is not always guaranteed. The addition of highly porous components like biochar into composting mixtures is applied nowadays in order to impro...

Recently, biostimulation has often been used to remediate oily wastes. The use of additional bioaugmentation to increase biostimulation efficiency is widely discussed in scientific and practical literature. In this study, three methods of bioremediation for oily waste containing 177.8 g kg-1 of hydrocarbons were used: the first one included biostimulation with 66.6 % compost (w/w) made from municipal waste, the second one combined biostimulation and one-time inoculation by hydrocarbon degrading strains, and the th...