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Currently, a big problem is increasing the production of residual oil, for which so-called tertiary recovery methods are used. One common tertiary recovery method is the use of surfactants. The use of biological surfactants instead of synthetic ones can solve the problem of increasing oil production and reducing the toxic impact on the environment. To obtain biosurfactants, two strains of Bacillus mojavensis and Bacillus amyloliquefaciens were used. These strains are characterized by the ability to produce biosurf...

Annually, a city with 500,000 inhabitants generates approximately 115.5 million cubic meters of wastewater. The treatment of this wastewater is efficiently managed using activated sludge, which comprises a diverse community of protozoa, infusoria, lower crustaceans, algae, and microorganisms. These organisms facilitate the effective treatment of large volumes of wastewater in treatment plants. However, the activated sludge proliferates by consuming organic materials in the wastewater, resulting in the accumulation...

Biochar is a highly porous pyrolysis product with a high specific surface area and adsorption properties, capable of adsorbing various compounds and formations, including ARG-containing MGEs. To assess the ability of biochar to adsorb MGE, a model experiment was carried out: in sterill flasks, the microbial community isolated from composts was cultivated on LB-medium, oxytetracycline and a mixture of heavy metals were added as factors potentially stimulating the production of MGE. Neither heavy metals nor oxytetra...

Soil contamination by polycyclic aromatic hydrocarbons (PAHs) is a widespread concern due to the high potential for human exposure through inhalation, ingestion, and dermal contact. In this study, we compared the efficiency of three different methods for PAHs extraction from model samples, including ultrasonic (US), mechanical shaking and purification with commercial QuEChERS kit (Shq), and ultrasonic extraction coupled with QuEChERS (USq) by gas chromatography coupled to a triple quadrupole tandem mass-spectromet...

The use of microalgae as carbon sequestrators requires optimization of the cultivation process. Growth curves for three types of microalgae were plotted. The growth was carried out at different temperatures 30 and 35 ? and with initially different concentrations of inoculum 30 and 3% in order to assess the possibility of growing crops at the lowest cost and with a high yield of a useful product. We used 3 strains of microalgae - potential sequestrators. The introduction of the inoculum at a dose of 30% led to an a...

Suppression of fungal phytopathogens is one of the serious challenges agriculture and forestry since they cause significant biomass and yield losses. One of the alternative methods of suppression phytopathogens is the use of biosurfactants, which are amphiphilic substances produced by many microbes. Bacteria of Pseudomonas genus are known to produce biosurfactants of the rhamnolipid class. These surfactants can possess antimicrobial and fungicidal properties. In this work, we studied the effect of various concentr...

Microbial communities associated with the plant rhizosphere play an important role in carbon sequestration, regulation of nutrient cycling, and the efficient functioning of the ecosystem as a whole. The diversity of microorganisms inhabiting the plant rhizosphere and their complex interactions with the host plant significantly affect the morphology, physiology, growth, development, and health of plants. At the same time, it is known that the soil microbiome diversity is affected by the type of soil, the type of cu...

Today there is a serious environmental problem - global climate change, and one of the main reasons for this change is the release of so-called greenhouse gases, and primarily raw carbon dioxide. One strategy to reduce the concentration of carbon dioxide in the atmosphere is to capture carbon dioxide from industrial emissions, since this is where the gas is found in high concentrations. In addition to physical and chemical methods, carbon dioxide capture is possible with the help of biological methods, for example...

Global climate change is one of the most acute environmental problems of our time. Among the methods of capturing CO2 in industrial emissions are chemical, physical and biological. The latter are based on the use of living organisms that absorb carbon dioxide in metabolic processes, and the subsequent use or burial of their biomass or their metabolic products. One variant of organisms used to capture carbon dioxide biotechnologically are microalgae. In the present study, the efficiency to capture carbon from the e...

Surfactants as detergents and emulsifying agents are used in various fields of industry and agriculture. These amphiphilic compounds contain hydrophobic and hydrophilic fragments and are usually produced by large-scale chemical synthesis processes. However, recently there is a request to develop biotechnological methods to obtain surfactants, since biosurfactants have many advantages above their chemical analogues - higher tolerance to extreme temperatures, pH and salinity, higher stability and lower or zero negat...

Biosurfactants produced by soil microorganisms are now widely used in various industries and are able to compete with synthetic detergents. However, in contrast with chemical surfactants, biological ones have disadvantage: it is difficult to obtain the product of high purity since the microbes usually produce a complex mixture of compounds, besides the cultural medium itself contains a lot of various components. Thus, the conventional scheme of lipopeptide isolation involves acid precipitation, extraction with the...

The problem of carbon dioxide emission from the soil is relevant, since it affects the reduction of the soil organic carbon pool, thereby reducing its fertility and productivity. In addition, carbon dioxide emissions from the soil are a significant contributor to the greenhouse effect. The aim of this work was to evaluate the mineralizing activity of the microbial community of the soil of a new point of observation of carbon dioxide emissions, located in the previously little-studied territory of Russia, using suc...

The number of registered cases of COVID-19 is increasing in the world, and some countries are reporting a second wave of the pandemic. Accurate and real time information about epidemiological situation is therefore urgently needed for managing decisions in the countries, regions and municipalities which are affected. Massive testing of viral presence in people’s saliva, a smear from the nose, nasopharynx and / or oropharynx, bronchial lavage water obtained by fibrobronchoscopy (bronchoalveolar lavage), as well as ...

Pyrolysis is considered as an effective and alternative way to treat organic waste and to obtain pyrolysis fuels as well as biochar. The latter may be used for different purposes in industry and agriculture, including use as a long term fertilizer. Biochar, after pyrolysis, is obtained in the form of powder; however from practical point of view it is better to use pelleted or granulated biochar since it significantly enhances biochar?s filed distribution. In our study we compared the efficacy of granulated and pow...

Pyrolysis is considered as an effective and alternative way to treat organic waste and to obtain pyrolysis fuels as well as biochar. The latter may be used for different purposes in industry and agriculture, including use as a long term fertilizer. Biochar, after pyrolysis, is obtained in the form of powder; however from practical point of view it is better to use pelleted or granulated biochar since it significantly enhances biochar?s filed distribution. In our study we compared the efficacy of granulated and pow...