
THE DESIGN OF MAGNETICALLY SEPARABLE BIOCATALYST ON THE BASIS OF GLUCOSE OXIDASE
(STEF92 Technology, 2019-06-20, Aleksandrina M. Sulman)
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Here we report immobilization of GOx on magnetic silica: (i) nanoparticles Fe3O4 with silica coating (Fe3O4-SiO2) and (ii) mesoporous silica with incorporated nanoparticles Fe3O4 (SiO2-Fe3O4) functionalized with amino groups using glutaraldehyde as a linker. Magnetic support based biocatalysts demonstrate high catalytic activity in D-glucose oxidation to D-gluconic acid at pH 5-7.5 and temperature of 30-50?C with the best activities of 95% and 94% for SiO2-Fe3O4-GOx and Fe3O4-SiO2-GOx, respectively. A comparison o...





