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The study of the properties and immobilization of various therapeutic agents on natural carriers is an actual task of medical biotechnology and bioorganic chemistry, since this allows creation of stabilized, biologically active drugs with controlled release, as well as a targeted site of action for use in various fields of medicine. Such therapeutic agents can be used as cleansing, antibacterial and (or) antioxidant treatments. The loss of biological activity of the materials, which contains various therapeutic ag...

The use of chemical crop protection products takes the lead in worldwide agricultural technologies. However, a significant part of pesticides does not achieve the goals, disperses in the environment, and accumulates in crops and food products. Targeted and controlled delivery of pesticides helps to decrease the overuse of chemicals due to the prolonged gradual release of active ingredients from matrices and maintaining of effective concentration in the application zone. The aim of this study was to evaluate the ef...

In this work hydroxyapatite (HAP) nanoparticles ranging in size from 30 to 150 nm of lamellar and rod-shaped form were obtained by controlled deposition in the presence of a stabilizer. HAP nanoparticle powder in different mass ratios was added to a mixture of biocompatible polymers (sodium alginate and PLGA), which were the dispersion medium of the emulsion. Water or an aqueous solution of sodium citrate was used as the dispersed phase. The internal structure of the matrices was studied by scanning electron micro...

The aim of this study was to investigate the effect of additional dietary chromium in various forms on the exchange of elements in rats. Ultradisperse chromium particles at a dose of 300 ?g/kg of feed contributed to significant changes relative to the control, including a 2.38 fold increase in calcium, a 15.00 fold increase in phosphorus and a 3.60 fold decrease in chromium. Relative to the control group, the introduction of 300 ?g/kg chromium chloride into the diet led to an 9.04 fold increase in P, 7.77 times mo...

This paper aims to study the effects of different densities on growth performance and feeding efficiency of common carp fry (Cyprinus carpio L.) growth in Recirculating Aquaculture Systems (RAS). Recirculating Aquaculture Systems (RAS) utilization have more advantage like: fish growth control, feeding management, diseases control, and water quality management and waste discharge in land-based aquaculture. The experiment was performed for 31 days, fish biomass being equally distributed into eight growth units. The ...

Glucose on an industrial scale is obtained in anhydride (at a temperature above 50 0C) or hydrate form (at a temperature below 50 0C). The crystallization process is conventionally divided into two stages: nucleation ? the formation of nucleus and their growth to crystals of technical dimensions. The most difficult stage is the formation of nucleus during the crystallization of hydrate glucose. Spontaneous formation of nucleus is slow and they have a fragile needle shape, from which it is difficult to grow technic...

This article presents the influence of pentosanases enzymes on the rheological parameters of the dough obtained from rye flour. The rheological characteristics of the rye dough were determined using the alveographic and consistographic methods. The functional properties of the main constituents of rye - starch, proteins and pentosans influence the baking characteristics rye flour. Rye does not contain as much gluten as wheat, and the quality of rye gluten is poor when it comes to retaining gases. This means breads...

This scientific research shows that some of the dietary fiber has a structural role in its plant sources, being insoluble in water. These are cellulose, hemicellulose and lignin. Some part of the food fibers have a repairing role and are soluble in water. This category includes pectin, gums, mucilage and hemicelluloses.[1] They are found in a lot of fruits like apples, bananas, tangerines, oranges, pears, plums, quine, grapes, kiwi. In this research it studied the protection mechanism of different types of fibers ...

Extracellular vesicles (EVs), as transporter of distinct bioactive molecules, can take part in different physiological mechanisms. Whereas a high number of studies have been published related to mammalian derived EVs, the existence of EVs in plants still is controversial. Plant EVs are poorly characterized, and their composition is largely unknown. Our knowledge of plant exosomes is limited, and we are far from understanding the biological functions of plant exosomes. The fusion of multivesicular bodies with a pla...

In study of 150 broiler chickens (Arbor Aires cross) was divided into five groups: control group, I - ultrafine particles (UFP) of Cr2O3 at dose of 50 ?g/kg of feed, II - 100 ?g/kg, III - 200 ?g/kg and IV - 400 ?g/kg. Growth-stimulating effect was confirmed by stimulation of protein metabolism (100 and 200 ?g/kg), growth of NO-metabolites (200 ?g/kg), triglycerides (100 ?g/kg, 400 ?g/kg). Functional significance of UFP Cr was expressed in activity of amylolytic enzymes in the blood plasma: on day 21 29.5% (400 ?g/...

Thin-layer chromatography is a useful and environmentally friendly analytical tool still with potential for the future. As a planar chromatographic technique has a wide spectrum of applications and it can be complimentary to other chromatographic techniques like, for instance, high performance liquid chromatography. Separation on thin-layer was performed for the very first time by Izmailov and Shraiber in 1937-1938. They called the new technique spot or drop chromatography and used it for separation of selected pl...

The concept of bioeconomy began to actively take shape in the world in the mid-2000s, when the Organization for Economic Cooperation and Development (OECD) and the European Commission (EC) began to develop programs to promote the bioeconomy in different countries. Documentation in the field of bioeconomy is a challenge for research, as official statistics report data on traditional sectors without distinguishing between synthetic and biological production. Therefore, indicators for the bioeconomic field are curren...

Microorganisms are ubiquitous in nature and possess unique biochemical possibilities to assimilate a vast array of available substrates and produce unique se metabolit and biotransformation pathways. Long-term exposure to elevated arsenic and antimony concentrations has led to the selection of specifically resistant autochthonous microorganisms. In the cultivable component of isolated bacterial microorganisms, Bacillus was identified as the dominant genus, which has been represented by nine species. Interesting ph...

MRI images contain a lot of subtle information related to various lesions which are difficult to be picked up by radiologists. Computer aided diagnosis CAD is a valuable tool to improve the ability of an average radiologist to diagnose the subtle lesions. This study uses the isolated foreground pixels in MRI images as a feature able to discern a stroke patient by a healthy one. Brain MRI image was divided into eight equal sectors. The isolated foreground pixels (i.e. pixels satisfying a neighborhood configuration ...

Bacillus megaterium is a bacterium that has been used for the industrial production of vitamin B12 (cobalamin), the anti-pernicious anaemia factor. Cobalamin is a modified tetrapyrrole with a cobalt ion coordinated within its macrocycle. Previous findings outline a potential way to develop B. megaterium as a cell factory for cobalamin production using cheap raw materials. In this paper, a novel strategy was implemented to investigate the possibility to use sunflower protein isolates and its enzymatic hydrolysates ...

Food processing wastes are the end products of the food processing industries which cannot be recycled or used for other purposes. They are the non-product flows of raw materials whose economic values are less than the cost of collection and recovery for reuse; thus they are discarded as a waste. These wastes pose increasing disposal and potential severe pollution problems and represent a loss of valuable biomass and nutrients. Avocado, mango and pomegranate processing significantly produces amount of waste (peels...

This paper compares four growth models (Logistic Model, Gompertz Model, Richards Model and Stannard Model) using the experimental data of wastewater treatment from a single sell protein production with Bacillus subtilis in order to describe bacterial biomass growth. One model was used to describe carbohydrate consumption during the bacterial fermentation. This study was carried at the Department of Biotechnology (Mendeleev University of Chemical Technology of Russia) as part of deproteinized sunflower meal bioconv...

Glucose toxicity is a well-established being that has been proven in higher vertebrates. The goal of this the present study was to elucidate the response of farming fish gibel carp Carassius auratus gibelio to waterborne sucrose in low (5.55 mM, LS) and high (55.5 mM, HS) concentrations for 21 days mimicking carbohydrate-enrich diet. An increase in blood glucose levels, body mass and hepatosomatic indices were the common sign for both concentrations of sucrose. The treatment with low concentration of sucrose provo...

Crystal dextrose is produced from solutions by enzymatic hydrolysis of starch. The process of crystallization of dextrose is the most complicated and responsible. The nucleation stage is particularly difficult. The common theory of crystallization connects the rate of nucleus formation with the surface tension of the solution. The purpose of the research is to activate the crystallization process by using surface-active substances (surfactants). Aliphatic alcohols: butanol, propanol, isobutanol, isopropanol were u...

Crystal dextrose is produced from solutions by enzymatic hydrolysis of starch. The process of crystallization of dextrose is the most complicated and responsible. The nucleation stage is particularly difficult. The common theory of crystallization connects the rate of nucleus formation with the surface tension of the solution. The purpose of the research is to activate the crystallization process by using surface-active substances (surfactants). Aliphatic alcohols: butanol, propanol, isobutanol, isopropanol were u...

For to determinate the DNA content and analysis of cell distribution in different phases of the cell cycle, flow cytometry was used. The exact measurements of a large number of nuclei by flow cytometry, fluorescently labeled by passing a laser beam (excitation), which marks each individual nucleus by fluorescence, allows a rapid estimation of the relative nuclear content of the DNA of the analyzed plants . Approximately 0.5 cm2 of foliar tissue is harvested, crushed very well, followed by incubation of the sample ...

For to determinate the DNA content and analysis of cell distribution in different phases of the cell cycle, flow cytometry was used. The exact measurements of a large number of nuclei by flow cytometry, fluorescently labeled by passing a laser beam (excitation), which marks each individual nucleus by fluorescence, allows a rapid estimation of the relative nuclear content of the DNA of the analyzed plants . Approximately 0.5 cm2 of foliar tissue is harvested, crushed very well, followed by incubation of the sample ...

Dill (Anethum graveolens), a condimentary and aromatic plant belonging to the Apiaceae family is widely used in the food industry due to its preservative and functional properties. Current study aimed to obtain ethanolic extracts of Anethum graveolens (seeds and fresh leaves) and to investigate these plant parts for their antioxidant activity (AA), total phenolics content (TPC) and profile of individual polyphenols, including: caffeic, ferulic, coumaric, rosmarinic, gallic, protocatechuic acids, rutin, resveratrol...

Dill (Anethum graveolens), a condimentary and aromatic plant belonging to the Apiaceae family is widely used in the food industry due to its preservative and functional properties. Current study aimed to obtain ethanolic extracts of Anethum graveolens (seeds and fresh leaves) and to investigate these plant parts for their antioxidant activity (AA), total phenolics content (TPC) and profile of individual polyphenols, including: caffeic, ferulic, coumaric, rosmarinic, gallic, protocatechuic acids, rutin, resveratrol...

The production of food waste covers all the food life cycle: from agriculture, up to industrial manufacturing and processing, retail and household consumption. Increasingly, industrial ecology concepts such as cradle to cradle and circular economy are considered leading principle for eco-innovation, aiming at ?zero waste economy? in which waste is used as raw material for new products and applications. The large amount of waste produced by the food industry, in addition to being a great loss of valuable materials,...

The production of food waste covers all the food life cycle: from agriculture, up to industrial manufacturing and processing, retail and household consumption. Increasingly, industrial ecology concepts such as cradle to cradle and circular economy are considered leading principle for eco-innovation, aiming at ?zero waste economy? in which waste is used as raw material for new products and applications. The large amount of waste produced by the food industry, in addition to being a great loss of valuable materials,...

The complex valorization of marine algae biomass is an important resource for many industries like pharmaceuticals and cosmeceuticals, as raw material for the extraction of bioactive principles (vitamins, pigments, sterols and collagen), cosmetics, biofertilizers and wastewater treatment. In the last years a special attention has been given to the use of macroalgae in the pharmaceuticals and dermato-cosmetics field. Algal growth is due to the amount of nutrients available in the marine environment. The aim of this...

The complex valorization of marine algae biomass is an important resource for many industries like pharmaceuticals and cosmeceuticals, as raw material for the extraction of bioactive principles (vitamins, pigments, sterols and collagen), cosmetics, biofertilizers and wastewater treatment. In the last years a special attention has been given to the use of macroalgae in the pharmaceuticals and dermato-cosmetics field. Algal growth is due to the amount of nutrients available in the marine environment. The aim of this...

Medical materials based on natural polysaccharides can be used as hemostatic sorbents and dressings. In this work, some samples of sponges had been obtained based on sodium alginate and chitosan. The concentration of sodium alginate in the solution, the ratio of alginate and chitosan were varied during an experiment. Polysaccharide cross-linking was carried out by inotropic gelation or by combining ionotropic gelation with ionic substitution. According to the literature, a chitosan have antibacterial properties ag...

Medical materials based on natural polysaccharides can be used as hemostatic sorbents and dressings. In this work, some samples of sponges had been obtained based on sodium alginate and chitosan. The concentration of sodium alginate in the solution, the ratio of alginate and chitosan were varied during an experiment. Polysaccharide cross-linking was carried out by inotropic gelation or by combining ionotropic gelation with ionic substitution. According to the literature, a chitosan have antibacterial properties ag...

Methods of parasite structures visualization using fluorescent probes are widely used in nowadays. The technological utilization of benzanthrone dyes is growing, but in biology studies they are still rarely used. Our studies show the advisability/expediency of using these dyes in investigation of helminthes structures. The aim of present study was to evaluate the novel synthesized luminophore AZP5 for efficient staining of trematodes for confocal laser scanning microscopy. All helminthes were previously preserved ...

Methods of parasite structures visualization using fluorescent probes are widely used in nowadays. The technological utilization of benzanthrone dyes is growing, but in biology studies they are still rarely used. Our studies show the advisability/expediency of using these dyes in investigation of helminthes structures. The aim of present study was to evaluate the novel synthesized luminophore AZP5 for efficient staining of trematodes for confocal laser scanning microscopy. All helminthes were previously preserved ...

With introduction of new and advanced technologies in beekeeping it is possible to design and use Internet of Things (IoT) sensor systems embedded in beehives. This provides the ability to collect heterogeneous data and to provide real-time remote monitoring. Complex prognostic models are applied after the data is manipulated and normalized. The obtained results are used to predict the processes occurring in beehives. For this purpose, the collected data must be divided into at least two parts for training and val...

With introduction of new and advanced technologies in beekeeping it is possible to design and use Internet of Things (IoT) sensor systems embedded in beehives. This provides the ability to collect heterogeneous data and to provide real-time remote monitoring. Complex prognostic models are applied after the data is manipulated and normalized. The obtained results are used to predict the processes occurring in beehives. For this purpose, the collected data must be divided into at least two parts for training and val...

The research carried out in the present paper aimed at monitoring and evaluating the technological process of obtaining the ecological cheese type ?PETROVA?, in the Maramures county area, Romania. To achieve this raw milk originating from domestic producers was evaluated in terms of fat content, acidity and density of this. After the final ecological cheese type product has been obtained, analyzes have been made for it?s physicochemical evaluation as follows: water content analysis, fat content analysis, salt cont...

The research carried out in the present paper aimed at monitoring and evaluating the technological process of obtaining the ecological cheese type ?PETROVA?, in the Maramures county area, Romania. To achieve this raw milk originating from domestic producers was evaluated in terms of fat content, acidity and density of this. After the final ecological cheese type product has been obtained, analyzes have been made for it?s physicochemical evaluation as follows: water content analysis, fat content analysis, salt cont...

The research carried out in this paper aimed to evaluate five types of beer throughout the technological flow of their production. The technological flow of producing the five types of beer has been monitored at all stages of it, starting with beer must, beer before filtering, BBT beer and bottled beer. To achieve this goal, the following physicochemical analyzes were carried out: color analysis expressed in EBC units, pH analysis, determination of bitter substances expressed in units of B.U. All the results obtai...

The research carried out in this paper aimed to evaluate five types of beer throughout the technological flow of their production. The technological flow of producing the five types of beer has been monitored at all stages of it, starting with beer must, beer before filtering, BBT beer and bottled beer. To achieve this goal, the following physicochemical analyzes were carried out: color analysis expressed in EBC units, pH analysis, determination of bitter substances expressed in units of B.U. All the results obtai...

Plants do not grow axenically in nature, but host a diverse community of microorganisms, termed as plant microbiota, colonizing different niches, both inside and outside their tissues, in the endosphere and ectosphere, respectively. These microorganisms are involved in major physiological processes, such as plant nutrition and plant resistance to biotic and abiotic stress factors. The aim of the research was to investigate the normal microbiota of rare plant species (listed with different degrees of danger in the ...

Plants do not grow axenically in nature, but host a diverse community of microorganisms, termed as plant microbiota, colonizing different niches, both inside and outside their tissues, in the endosphere and ectosphere, respectively. These microorganisms are involved in major physiological processes, such as plant nutrition and plant resistance to biotic and abiotic stress factors. The aim of the research was to investigate the normal microbiota of rare plant species (listed with different degrees of danger in the ...

Endophytic bacteria can improve the growth and development of plants, and also prevent the growth or activity of plant pathogens, reduce the aggressiveness or causative agent. Endophyte bacteria can be specific depending on the host. The properties of the microorganisms colonizing tissues of various plants can vary greatly. Therefore, the study of endophytes present in plants is of great interest because of their possible subsequent use in order to increase the productivity of plants and protect them from pathogen...

Endophytic bacteria can improve the growth and development of plants, and also prevent the growth or activity of plant pathogens, reduce the aggressiveness or causative agent. Endophyte bacteria can be specific depending on the host. The properties of the microorganisms colonizing tissues of various plants can vary greatly. Therefore, the study of endophytes present in plants is of great interest because of their possible subsequent use in order to increase the productivity of plants and protect them from pathogen...

Fennel (Foeniculum) is a member of the Apiaceae family, the most cultivated fennel cultivars belong to subspecies vulgare. This paper aim is to obtain essential oil from Foeniculum vulgare (FEO) seeds cultivated in the western part of Romania and to characterize the FEO in terms of chemical composition, herbicidal and antibacterial properties. Fennel essential oil was obtained by water vapour entrainment and the chemical composition of the oil was determined by GS-MS.The herbicidal effect was studied against weed ...

Fennel (Foeniculum) is a member of the Apiaceae family, the most cultivated fennel cultivars belong to subspecies vulgare. This paper aim is to obtain essential oil from Foeniculum vulgare (FEO) seeds cultivated in the western part of Romania and to characterize the FEO in terms of chemical composition, herbicidal and antibacterial properties. Fennel essential oil was obtained by water vapour entrainment and the chemical composition of the oil was determined by GS-MS.The herbicidal effect was studied against weed ...

More and more scientific research studies have shown that plant extracts can act as a potential mediator for the synthesis of nanoparticles. The plant extracts are efficiently used in the synthesis of ecological metallic nanoparticles such as silver, gold, zinc, copper, palladium, platinum, cobalt, titanium, etc. Furthermore, it has been proved that plant extract-based nanoparticles are a potential remedy for various human and plant diseases. The use of nanotechnology in agriculture has been intensely debated and ...

More and more scientific research studies have shown that plant extracts can act as a potential mediator for the synthesis of nanoparticles. The plant extracts are efficiently used in the synthesis of ecological metallic nanoparticles such as silver, gold, zinc, copper, palladium, platinum, cobalt, titanium, etc. Furthermore, it has been proved that plant extract-based nanoparticles are a potential remedy for various human and plant diseases. The use of nanotechnology in agriculture has been intensely debated and ...

The paper gives a brief overview of the possibility of using microorganisms in ecological farming. Increasing the world's population is a serious challenge in the future to ensure food for mankind. That is why the scientists are looking for ways to increase agricultural production in a sustainable way and preserve the quality of the environment. The current concept of agriculture tends towards a unifying vision in which crop plants, microorganisms and agricultural land form a unity. Within this complex system, the...

The paper gives a brief overview of the possibility of using microorganisms in ecological farming. Increasing the world's population is a serious challenge in the future to ensure food for mankind. That is why the scientists are looking for ways to increase agricultural production in a sustainable way and preserve the quality of the environment. The current concept of agriculture tends towards a unifying vision in which crop plants, microorganisms and agricultural land form a unity. Within this complex system, the...

Cabernet Sauvignon variety is one of the most widespread grapes varieties for winemaking grown in Romania. By application of clonal selection, five clones of Cabernet Sauvignon have been obtained and omologated in Romania. The present study aimed to characterize the quality of wines obtained from three Cabernet Sauvignon clones, namely Cabernet Sauvignon 30 Vl., 131 St. and 54 Mn. in the Valea Calugareasca viticultural area, considering the extension of their culture and market potential. The experiments were carr...

Cabernet Sauvignon variety is one of the most widespread grapes varieties for winemaking grown in Romania. By application of clonal selection, five clones of Cabernet Sauvignon have been obtained and omologated in Romania. The present study aimed to characterize the quality of wines obtained from three Cabernet Sauvignon clones, namely Cabernet Sauvignon 30 Vl., 131 St. and 54 Mn. in the Valea Calugareasca viticultural area, considering the extension of their culture and market potential. The experiments were carr...