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The oxygen plasma-based bonding of a liver-on-a-chip platform has been optimized for immunostaining and RNA extraction during differentiation of induced pluripotent stem cells into hepatocytes on-chip. The device is a structural and fluidic mimicry of the liverпїЅs lobules, consisting of a double layer of polydimethylsiloxane (PDMS) bonded to glass. This bonding has been adjusted in terms of power, exposure time and post-exposure baking to make the device separable from its glass slide, making cells cultured insid...

The most studied and promising for the biotechnological production among extreme halophiles are archaebacteria Halobacterium salinarum. Food additives for humans and animals based on these halobacteria have an immunostimulating, antioxidant effect, can be used in complex therapy of viral diseases, and contain biologically unique active substances, vitamins and microelements. High-price hydrolysates and enzymatic hydrolysates of high-quality raw materials are used for H. salinarum cultivation. This causes a high co...

Starch is widely used as a biodegradable component in the production of modern packaging materials. Determination of starch is necessary to control the stage of production of starch-containing materials, as well as to assess their biodegradability. The main process leading to the destruction of biodegradable composites is the biological degradation of starch under the influence of enzymes when starch-containing materials are located in the natural environment. Two types of starch-containing packaging materials wer...

Drought and associated effects can cause serious damage to crop maize. Breeding drought tolerance by the development of new hybrids, aims at identifying some accurate screening techniques. The objective of this study was to evaluate the effect of the hydrological conditions (with stress - drought year and without stress - normal year) on the yield and other traits for six maize hybrids grown at ARDS ?imnic, Craiova and to investigate drought tolerance of these hybrids using several stress tolerance indices. Analys...

The aim of this study was to analyzed the effect of calcium ions from gluconate and lactate salt at the level of 100, 150 and 200 mg/100g addition on empirical dough rheological properties from the wheat flour type 1250. Dough empirical rheological properties were analyzed using the Farinograph, Amilograph, Glutograph, Alveograph, and Rheofermentometer devices. These devices allow us to obtain complete informations about dough rheological properties during mixing, pasting and fermentation process of wheat flour do...

Wet ageing is the most commonly used ageing sistem by the meat industry to improve tenderness, which results in a more homogeneous product with high consumer acceptability. The aim of this study is to determine the influence of wet ageing duration on beef quality characteristics, at refrigeration temperatures (0 - 5пїЅC). The meat samples were kept under refrigeration conditions for 2, 7, 12, 17, 21 days. After each period, there were monitored: the textural parameters (hardness, adhesiveness, springiness, cohesiv...

Natural Killer (NK) cells are large granular cytotoxic lymphocytes that play a role in the innate immune response against tumour cells and virally infected cells. In pathogen-free chickens, these cells are thermolabile at 37oC and resistant to treatment with anti-B or anti-T cell anti-sera. This is in contrast to humans and rats, where NK cells are resistant to temperature manipulation for 30 or 60 minutes. While some reports suggest that NK cells are adherent and phagocytic, others suggest they are non-adherent a...

The bioactive compounds in apples and pears might confer many health protective benefits by improving oxidative stress, on the other hand in case of higher than the admitted values, Heavy metals could affect health. Apple and pears are members of the Rosaceae (Rose) family, known for their pomaceous fruits. The aim of this study was to describe the quality traits, the level of antioxidant activity and polyphenol content of some apples and pears available on the Romanian market as well as the heavy metals concentra...

The demand on functional food products enriched with probiotics to get health benefits is recently high. On the other hand, the use of probiotics in animal nutrition has been shown to have significant economic and agricultural effect. Animal components are substituted by plant raw materials as a base for probiotic microorganisms fermentation nutrient media. Cereal raw materials and processed products are of interest. It is well known, that the costs of raw materials and its preprocessing influence on the costs of ...

ItпїЅs well known that sea buckthorn berries are rich in vitamins, carotenoids, flavonoids, and other biologically active components with significant therapeutically properties.The main polyphenolic compounds from sea buckthorn fresh juice were identified and quantified using HPLC-DAD method and the ?-carotene content was determined using the spectrophotometric method.The extractions were performed using four different solvents. The sea buckthorn juice was fractional extracted using: diethyl ether (Fraction A), me...

The Romanian eggplant growers get important productions in protected spaces, besides optimum natural periods. The average yields obtained from crops of grafted eggplants are with approx. 25% higher compared with productions of ungrafted culture. The experience was conducted at the Horting Institute Bucharest, Romania. The grafted and ungrafted eggplant plants were obtained and maintained during vegetation period according to the classic technologies, in some specialized greenhouses: a greenhouse specialized in pro...

The carbohydrate (2.9-7%), vitamins (A, B, B2, B6, C), salts of important mineral elements (K, P, Fe, Ca, I, Mg) and organic acids (0.5-1.5%) content from tomato fruits is very important. The tomato is widely cultivated all over the world and a very popular and important crop in Romania. The grafting is considered an important technique for the sustainable greenhouse vegetable production. This research was conducted on import tomato hybrids appreciated in south side. Two Lycopersicon esculentum rootstocks - пїЅEmp...

The purpose of this research was to improve the nutritional and sensory properties of butter biscuits by addition of grape pomace. The pomace derived from two grape varieties, Pinot Noir and Italian Riesling, was dried at 60пїЅC for 8 hours daily, three days in a row. In the butter biscuits recipe, the wheat flower was replaced with three doses of grape pomace (10, 20 and 30%) added in the form of powder. A control sample, without any addition of grape pomace, was also prepared. The biscuits were analyzed in terms...

The purpose of this study was to determine an optimal variant for producing skimmed stirred yoghurt with chia powder. Three variants of product with chia powder at levels of 0.3%, 0.5%, respectively 0.7% and two control samples have been manufactured. Chia was used due to its remarkable content in fibre, ?-3 and ?-6 fatty acids, polyphenols, which are essential compounds for a healthy human body. The physicalchemical parameters (acidity, pH, dry matter) and rheological properties of yogurts samples were determined...

The paper proposes an evaluation of the flavor potential of the Pinot Noir wine, in whose production technology maceration-fermentation enzymes were used and their influence in the context of variable temperature parameters. The GC_FID methods used led to the identification and quantifying of esters such as Ethyl Octanoate, Ethyl Decanoate, Diethyl Succinate, Ethyl Lactate, Isoamyl Acetate, Ethyl Acetate, Ethyl Formate, that combined led to the conclusion that maceration-fermentation enzymes and temperature contri...

In this paper were realized some researches concerning the grinding process of plates used by the sintering process DMLS. This plates are used in Direct Metal Laser Sintering process like support necessary for the powder deposing and for the final pieces realized by sintered process. The material used in DMLS process necessary for dental crowns manufacturing is a Co-Cr alloy. Co-Cr alloy is a super stainless steel with good mechanical and corrosion resistance. The material used for plates is a Fe-15%Cr martensitic...

Nasal cheese is a smear ripened traditional Romanian cheese produced in a natural cave near Taga commune, Cluj County. The effect of varying heat treatments (65 or 80 пїЅC for 20 and 5 minutes respectively) and salting methods (dry or brine salting) on bacterial viability and biochemical properties in Nasal cheese were investigated. Brevibacterium linens, a corynebacterium produce a characteristic "sulphur" aroma associated with such cheese. Therefore, this study also investigated the profile of volatile compounds...

Particle size is an essential concept in flour milling which influences dough rheological properties and food product quality. The grape seeds flours (GSF) obtained by further grinding grape seeds vary in particle size and differ in chemical composition. Grape seeds selected from red wine pomace and wheat flour of a low extraction rate of 480 type were used in samples formulation. The objective of this work was to investigate the influence of different particle sizes and addition levels of grape seeds flour (GSF) ...

The present work aimed to investigation of influence molecular weight of chitosan, method of silver nanoparticles producing and immobilization, amount of cross-linking agent on antibacterial activity. Silver was incorporated in the matrix in two ways: 1 пїЅ by the recovery of silver ions from AgNO3 solution; 2 ? by addition to hydrogel the silver nanoparticles, obtained previously by microbiological synthesis. The molecular weight of chitosan shows strong influence on internal structure of matrices and on release ...

To increase the efficiency of conversion of plant raw materials, a search for new enzymes is required. The hydrolysis of xylan using industrial xylanases is widely used in the production of bleached cellulose. However, an increase in activity, selectivity and thermostability of enzymes is relevant for modern biotechnology. Recently, the sources of bacteria that have important practical significance are very diverse: from sea depths to volcanoes. But there are also accessible habitats, for example deposits of waste...

Enzymes are major components of organism defense against toxic chemicals in their environment. Despite the passage of more than 200 million years of life presence these enzymes now play an important role in detoxifying chemicals man-made addiction and it may be a useful biomarker. Lactate dehydrogenase (LDH or LD) is intracellular enzyme year found early in all living cells (animals, plants, and prokaryotes). LDH catalyzes the conversion of pyruvic acid to lactic acid and back, as it converts NAD + to NADH and bac...

This study aimed to investigate: i) the chemical composition and ii) the antimicrobial properties of the essential oil isolated from Lavandula angustifolia пїЅSevtopolisпїЅ cultivated in western Romania. The essential oil, obtained by steam distillation, was evaluated by GC/MS. The inhibitory effects of Lavandula angustifolia пїЅSevtopolisпїЅ essential oil were tested using the Kirby-Bauer agar disc diffusion method against five common food-related bacteria: Staphylococcus aureus (ATCC 25923), Pseudomonas aerugino...

In the present study, low-fat muffins in which butter has been replaced at different levels (50% and 100% respectively) with pumpkin seeds powder were prepared. The valuable content of the pumpkin seed powder in proteins, fibres, vitamins, minerals and its good sustainability are important reasons for adding this ingredient in the food products. The physical-chemical parameters: weight, moisture, moisture loss, volume and specific volume were determined during storage period. Minerals content was carried out by us...

The literature mentions that marine algae biomass is an important resource of mineral elements, polysaccharides (agar-agar, alginats), proteins and vitamins. The superior valorification of this biomass represents a highly important resource for the pharmaceutical industry, textile and dyes industry, nutrition industry and for soil fertilization. At the Romanian Black Sea Coast the macro-algae biomass envelopment is reported mainly in summer and is registered especially by the green macro-algae group; thus the larg...

The marine ecosystem has been the cradle of life on Earth, and the entire evolution of human populations was related to the sea. Always present, in most great civilizations, from economy to art, the marine ecosystem always played a special and complex part in the human conscience and sole. Our article focuses on the relationship between certain factors of the marine ecosystems and both wellness and pathology of the eyes. Just like the tide of the sea goes both ways, so do the effects of the sea water and its assoc...

Soil organic matter is fundamental for the long-term sustainability of agro-ecosystems. An experiment was conducted on the experimental plot aiming to determine if organic matter represented by mulches with wool wastes and wood chips could induce changes in soil microbial community composition and activity and effects on improvement of soil suppressiveness to plant pathogens from the genus Fusarium. The soil was Technosol and the organic culture of edible roses was planted in 2015. In the summer of 2016 the soil w...

The main goal of our paper was to determine the consequences of selenium intake (0.2 ppm as selenium selenite) on mineral profile of some broiler chicken (Ross) tissues/organs. Scarcity of studies on reference ranges for various minerals in different tissues was the secondary reason of our study. Our results highlighted significant variations on minerals content from studied samples: blood пїЅ Cu, Mn, Fe; faeces пїЅ Cu, Mn, Fe, Mg; muscular stomach пїЅ Fe, Zn, Ca; liver пїЅ Fe, Mg, Ca, Na; kidney пїЅ Cu, Mg, Ca, N...

Nowadays the great attention is paid to the cultivation of different mammalian cells. They can be used, for example, for vaccines, monoclonal antibodies or viruses production, in regenerative medicine, diagnostics, for clinical trials and etc. The application of modelling and simulation tools for these purposes is very convenient and perspective in order to decrease the experiments and therefore the total cost for the investigations. In this article, the process cultivation of CHO cells is considered in hollow-fib...

Fibre formation is one of the key issues in plant biology. The walls of plant fibres consist of several layers with different structural organization, chemical composition and properties. The morphology of the secondary wall largely defines the physical and physicochemical properties of plants. In spite of the progress in knowledge and technical ability, various problems concerning fiber formation remain basically unsolved. The new data are obtained by visualization of birch Betula pubescens and spruce Picea abies...

Fruit juices are consumed worldwide, not only for their flavour, taste, and freshness, but also due to their beneficial health effects when are consumed regularly. Being relevant sources of vitamins, minerals and polyphenolic compounds many people are becoming aware of the importance of consuming them in their daily diet. The citrus juices, lemon, lime and grapefruit juice is the most appreciated and used because of its flavour and high content of vitamin C. The purpose of this study was to analyze and compare som...

Developing lipid products with colloidal structurally and functionally modified properties, others than the starting base, playing a structural role in the integrated product and the role of mycelia carriers to ensure a health status is relatively recent and it is the result of accepting the idea that foods play a determining role in the prophylactics and therapy of certain conditions. The paper approaches two main aspects: 1 пїЅ methods of modifying lipids (simple stir, hydrogenation, interesterification, fractio...

Cereals and by-products of its processing are known to be applied for lactic acid bacteria fermentation. Wheat grains containing starch, protein and a lot of growth factors seem to be the rich nutrient media for some types of microbes. On the other hand, the niglible number of lactobacilli can produce amylases and proteinases (for native gluten decomposition), so at least the partial hydrolysis of substances mentioned is needed. The complex processing of wheat flour with amylases and proteases was shown to increas...

The purpose of the research paper was to study the sensorial and physic-chemical characteristics of the `Carpinet` pie produced as a traditional ecological food product in a Romanian village, in the West part of the country. The technological production process of the `Carpinet` pie followed a traditional receipt known as a local bakery product. All the raw materials and ingredients used in the technological process according to the traditional receipt had organic origin as ecological ingredients. The sensory exam...

The researches presented in this paper aim to study the production technology of traditional ecologic meat products sausage type and their physic-chemical and sensorial characterization. The studied meat products sausage type were the traditional пїЅVirsliпїЅ produced in the Romanian village Pui by local producers. The first part of the researches involved the production technology, using organic raw materials and ingredients. Also the production technology followed a traditional recipe used only in this region / ...

Zizyphus lotus L. is a medicinal plant used in traditional medicine of many countries like sedative, analgesic, tonic and anti-inflammatory. The decoction of roots is used by people with diabetes as a hypoglycaemic agent. Zizyphus lotus is also used to treat the digestive tract and liver. The leaves of Zizyphus lotus are used against the bites of the vipers in the Sahara and the fruits are recommended in the treatment of sore throats and respiratory affections. Fruit species of the genus Zizyphus, with great poten...

The argan tree (Argania spinosa) plays an irreplaceable role in the ecological balance and in the preservation of biodiversity. Thanks to its powerful root system, it contributes to the maintenance of the soil and makes it possible to fight against the erosion of water and wind which threatens desertification a good part of the region. The limits of the argan tree are fixed by the climate. The argan tree is a thermoxerophytic species, whose distribution area overlaps with both semi-arid and arid bioclimates. In ad...

Biochemical composition and microbiological conversion potential of broccoli crop residues was the objective of this research; Florets, leaves and stalks of broccoli were dried and stirred separately. The powders obtained were mixed together in ratio 12:8:80 % respectively, that corresponded to the distribution of these components in farm residues. The biochemical composition of the resulting mixture was evaluated. The identification and quantification of simple carbohydrates of broccoli juice were done with HPLC....

On the last two years, there was carried out some research work on the medicinal plants from wild flora. All the research was done on the area of Nera river basin because this region is a protected area and has a very large biodiversity. As far as we know on this area there are over 1000 species of superior plants classified on 98 families. One of this plants growing on this region is Oenothera biennis. One of this plant pathogens is powdery mildew produced by one of Erysiphe genus species. Regarding to the work m...

Cereal farming is an important sector of agriculture in Latvia, which determines the development of crop farming. In 2015, 22.5 thousand farms were engaged in cereal production in Latvia. Of the total output of cereals, 64% were produced by intensive farms with an area of more than 200 ha, which accounted for a few percent of the total cereal farms. The cereal sector has developed in Latvia after joining the European Union (EU) owing to direct support payments and export opportunities. It is important to project t...

The purpose of this study is to evaluate the mineral content, total antioxidant activity and total phenolics content of pumpkin flesh and seeds from two varieties of autochthonous pumpkins Garden pumpkin (GP) and Styrian pumpkin (SP) purchased from the agricultural marketplace. The investigated samples consisting of Garden pumpkin flesh (GPF), Garden pumpkin seeds (GPS), Styrian pumpkin flesh (SPF) and Styrian pumpkin seeds (SPS) were subjected to mentioned investigations, as follows: the mineral content was analy...

Animal bodies (mammalian and human body) show a great capacity for adaptation, both to the fluctuations of environmental factors and also regarding the nutrition, increasingly modified. General Adaptation Syndrome (GAS) of organisms, began to be studied in animals and also in humans. In human bodyes, GAS is studied because a number of disease with great significance psychologically, physiological but especially medical, appeard. In both categories of bodies, GAS is investigated by the presence of markers that high...

Worldwide, 81% of cultivated soybeans is genetically modified. Soy is just one of the 68 products on the European list of approved GMOs for import, along corn, rapeseed, cotton. Cross-border exchanges lead to the introduction of GMOs on the market, requiring careful monitoring of food with the help of appropriate testing techniques. According to the EU legislation, the labeling of foods containing genetically modified organisms is mandatory, therefore being set a limit of 0.9% per ingredient. The research presente...

These researches were conducted in the greenhouses resort didactics of the faculty of Horticulture and Forestry in Timisoara, using cuttings of Parthenocissus quinquefolia that were purchased from the mother plant from the same location. Four substrates were used for rooting namely sand, peat, perlite, perlite + peat, the cuttings were planted and treated with rooting substance of Atonic and Radistim. During the study was monitoring the rate of rooting of cuttings, aiming at influence of substrates and rhyzogene s...

This paper shows laboratory researches in order to extract arsenic and cadmium from the polluted soils. Research is based on preliminary studies regarding the usage of microorganisms which were selected from the same sampling points of soil assays for decontamination of soil polluted with heavy metals (As and Cd). The soil samples are subjected to the cleaning process with different extraction solutions (9K medium and chemical solutions) stirred 200 rpm for 72 hours at a controlled temperature (32 пїЅC). During th...

Food safety has become a component of food quality and arises from the need to protect consumers. This means that products to be sold, must be safe, a requirement which is found both in EU Directions and National Legislation. The Food Hygienic General Direction 93-43- CEE stipulates that the food companies including those of fresh fruits, should create a planned and well informed system called пїЅThe Planning and Achievement of Safety productsпїЅ. The system allows a self-control of their own products based on Haz...

Recycling vegetable and animal wastes from households, livestock farms, vegetable farms, industry and timber processing, mushroom cultivation, and so on and using them for fertilizing vegetable crops in various forms (mixtures with organic and inorganic matter, compost and green fertilizer) is a permanent concern of researchers and farmers worldwide.In the glasshouse, it was studied influence of four types of compost (composted leaves, mixture of pieces of grape woods and tree branches, waste mushroom compost and ...

The effect of mastitis on the content and composition of the milk lipids is not fully known yet, the literature presenting numerous contradicting data. In this work it was aimed to emphasize the modifications occurred at the level of the composition in fatty acids and cholesterol, as well as to establish the level of peroxidation of lipids in the milk resulting from cows with sub-clinical mastitis as compared to the milk resulting from healthy cows. Thus, within the researches carried out in 2016 in Olt county the...

The purpose of this paper is to determine the chemical composition of meat from two species of fish (Cyprinus carpio carpio - Linnaeus 1758 - carp and Svizostision lucioperca - Linnaeus 1758 - pike-perch), fresh and preserved in different ways at the end of the season growth, autumn and spring after the cold season. The changes in the quality of preserved fish meat through refrigeration, salting, freezing and smoking compared to the fresh, one have been analyzed so that the consumer benefits from safe food and a r...

In the study presented were used Pandorea jasminoides cuttings, they were planted in three different periods and treated with rooting stimulants. It was followed the rooting rate, depending on the period of the planting cuttings and depending on rooting substance that has been used. The experiment was conducted in a closed greenhouse in the Faculty of Horticulture and Forestry in Timisoara. Considering the three periods of initiation of the process of rooting cuttings, Pandorea jasminoides averages were recorded c...

The skin is covered with a hydrolipid film that, depending on the area of the body, comprises secretions from sebaceous glands and from apocrine and eccrine sweat glands. Decomposition products from corneocytes and cornification (cellular debris and stratum corneum lipids) in the process of being shed are also present. This film provides a natural defense against pathogenic organisms but also attracts dirt and pollutants from the environment. Thus, periodic cleansing to remove debris, dirt, and odor is essential t...