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Currently, in order to respond to the needs of the human population and food security issues worldwide, it is necessary to implement strategies for recovery and transformation of waste from the food industry into a circular system of production and consumption. Fruit waste such as peels and seeds have a high waste rate worldwide. These wastes have a high nutritional content, suggesting a potential for use in the food industry. Pome fruits (apples, pears and quince) belong to Rosaceae family, have tough central cor...

Conventional wastewater treatment plants (CWWTPs) play a key role in reducing the environmental impact of urban wastewater. But nowadays, this role goes even further with climate change, water scarcity, and the current energy crisis. After the thickening of the secondary sludge, a wastewater flow is generated, which is normally recirculated to the plant head for further treatment. This work aims to use secondary thickener wastewater (STW) as a culture medium for Chlorella vulgaris, Neochloris oleoabundans and Scen...

Legumes are a unique source of protein for food and feed industry. Legumes synthesize protein valuable for living organisms using atmospheric nitrogen which they can fix due to their symbiosis with root nodule bacteria. The formation of plant-microbial symbiotic systems depends on complementary interaction of signaling molecules of both symbionts. Rhizobia signaling molecules are lipochitooligosaccharides, so called Nod factors. The synthesis of core part of Nod factor molecule is determined by nodA, nodB and nodC...

To prove adverse impacts exerted by drinking water and to assess regularities in occurrence of health disorders caused by contamination burden on the body, experts from the Center performed complex chemical-analytical and medical-biological examinations of children who lived on an exposed territory and consumed drinking water with chemical contents exceeding hygienic standards: N-nitrosodimethylamine, by 1.6 times; chloroform, by 5 times; dichlorobrommethane, by 26 times; and children who lived on a reference terr...

Legumes are nutritious ingredients that can be used in bakery industry. However, in order to minimize their antinutrients contents and unpleasant flavor, germination can be applied. The aim of the present paper was to investigate the proximate composition and minerals profiles of composite flours made of refined wheat flour and germinated legumes (beans, lentil, soybean, chickpea and lupine) in different levels. The results showed an increase of protein and ash contents as the addition level was higher, while a re...

Environmental pollution in industrialized regions has a negative impact on public health status. Excessive accumulation of heavy metals in the human body induces disturbances in the adaptation processes to changes in the quality of the environment, including changes in the immune, humoral and nervous regulation, which can lead to the early development of ecologically caused pathologies in children. Purpose of work: to study the peculiarities of the immune profile of the child population in an industrialized region...

Malt is the essential raw material for beer production. Currently, in Europe the most used cereal for beer is barley due to its many advantages in the manufacture of malt. Recently, research has intensified to replace this raw material for beer with other cereals or pseudo-cereals. These include buckwheat which does not contain toxic prolamins to celiac disease patients. Buckwheat is an annual plant of Polygonaceae family and is produced in many countries for its characteristics such as high economic value, easily...

Peptide-drug conjugates are therapeutic agents that combine one or more drug molecules with a peptide through a biodegradable linker. Even if the peptides used in synthesis of such conjugates have a smaller pharmacological action than the small molecule of organic drug, they easily penetrate the membranes and then bind to receptors. Peptide-drug conjugates are used in various fields, such as: (i) anticancer therapy; (ii) antimicrobial application; (iii) anti-inflammatory application. In the last decades, researche...

Chemical compounds of natural origin have high biological activity, complex effects on the human body, and safety for health. Preparations based on natural plant raw materials affect the body's adaptive capabilities, acting as factors of nonspecific defense and cofactors of the immune system. The aim of the study is to evaluate the effect of phenolic constituents of plant origin on the phagocytic activity of peripheral blood cells in vitro. Materials and methods. In vitro study of the effect of phenolic constituen...

In this work, experiments have been carried out to study the impact of various stress factors present in the culture medium on the growth and bio-compound production for the microalgae species Porphyridium purpureum. Samples of microalgae have been grown under various stress conditions in a thermostated incubator under mixing and artificial light. Biomass production was determined by removing the cells through centrifugation followed by lyophilization. For exopolysaccharides isolation, the precipitation method wit...

UNESCO’s Global Geopark Network reflects the importance of geo-heritage for society. The wide range of characteristics and the varied connections to the local ecology and culture are significant for their individual management. The aim of this article is to use comparisons between Geoparks as a step toward a generic, conceptual model that can be adapted and used to understand the crucial, functional relationships between system variables and how these variables can be optimized for stainable management. A general ...

Oilseed processing technologies include a sequence of intensive multiple heat treatments of seeds, oil and intermediate products. The optimization of these processes largely determines not only the price, but also the quality of the final products. Heat and mass transfer in multiphase multicomponent media are very complex. It is not yet possible to build adequate models of all processes theoretically. The thermophysical properties of the media under study change significantly with heating and changing pressure. Ne...

The immobilization of glucose oxidase (GOx, enzyme of oxidoreductases class) was carried out on non-magnetic (SiO2, Al2O3) and magnetic mesoporous oxides (Fe3O4-SiO2, Fe3O4-Al2O3). Magnetic supports were synthesized by in-situ crystallization of magnetite nanoparticles in mesopores. The surface of the support was preliminarily modified with amino groups and the glutaraldehyde was used as a linker for covalent binding of the enzyme. The supports and biocatalysts were fully characterized using a combination of physi...

Greenhouse heating during cold seasons is costly as it is high energy consuming. The use of latent heat stored during sunny periods for greenhouse heating could be an interesting alternative in several regions worldwide as in Tunisia. Thus, new agronomic/climatic approaches were developed to produce several crops such as tomato in a sustainable way. In this context, maturity and yield of tomatoes cultivated inside a greenhouse equipped with a new solar air collector with latent storage (IGHLS) were studied in comp...

Detection of nickel ions in various foods, especially fruits, is very important for the prophylaxis of patients with allergic contact dermatitis to this heavy metal. Currently, there are a limited number of rapid methods for identifying nickel ions, so it is necessary to develop new biosensors in this area, by improving selectivity of ligands and utilization of immobilization elements suitable for nickel ions. The mentioned elements are very important because they can increase the selectivity, sensitivity and can ...

Porous supports including oxides and polymers have been employed for enzyme immobilization for many years. In recent times, the research focus has been placed on hierarchical structures, dependencies on pore sizes, and the use of magnetic porous oxides for magnetic recovery. The presence of pores or cavities where enzymes could locate to create an optimal degree of crowding achieved in cells is not a prerequisite, but it could significantly enhance the catalytic activity of biocatalysts. For the last five years, t...

Food biotechnology is one of the green and fast developing segment of the industry among the others dealing with renewable plant raw materials processing and new functional food production to improve human health in deteriorating environmental situation. Annually, the number of studies aimed at proving the prebiotic properties of certain substances or compositions is increased, which leads to a huge accumulation of scientific information. The divergence in techniques for prebiotic activity assessment cause the fac...

Antibacterial preparations are widely used in agriculture worldwide to achieve a wide range of effects, including therapeutic and preventive ones; they are also used as forage additives to stimulate animal growth. Since the issue related to the development of antibiotic-resistant microorganism strains has become vital, the use of antibiotics in agriculture is strictly regulated. Thus, in the European Union (EU) countries and Eurasian Economic Union (EAEU) countries, antibacterial preparations can be used in treatm...

The aim of this work is to optimize the growth parameters of D. salina by altering nutritional factors, in order to maximize production of antioxidants and lipid fraction by the microalgae strain. For this purpose, the concentrations of different salts in the growth medium were varied. The biomass obtained was analyzed quantitatively and qualitatively in order to determine biomass concentration and bioactive compounds concentrations in respect to the growth medium composition. Pigments were extracted from the drie...

A severe problem affecting urban areas is a large number of stray animals, either r eject- ed by their owners or born in the wild. These animals face the problem of malnutrition, as it is difficult for them to obtain food without human help in urban conditions. The fact that shelters are overcrowded is not suitable for these animals as well well. In o rder to protect them from the tragic death of starvation, the authors decided to de- velop an intelligent system for feeding stray animals. The system makes it possi...

In the current work, the process of fermentative conversion of animal waste and non-hydrolized plant materials (peat) using food waste and ascorbic acid salts as a stimulants is described. The amino acid composition of the biofermentation products was estimated. The possibility of the target biosynthesis for the production of fertilizers and feed additives with the pre-determined properties was recognized. It was shown that the additive of bread making waste with the concentration of 10 wt. % increases the amino a...

Inhibition of the coil-helix transition of gelatin and retardation of the gelation process of the system as a whole is an important scientific problem. The solution to this problem will make it possible to maintain the properties of gelatin solutions for a long time without changing their rheological characteristics. In this work, by the method of dynamic mechanical analysis, the dependences of the time profile of gelatin solutions depending on the concentration of 10, 13 and 16 wt.%, as well as the different diss...

The effectiveness of rhizobia-legume symbiotic interaction is under the genetic control of macro- and microsymbionts. Both symbionts exhibit high level of genome/genome specificity interaction which is reflected by symbiotrophic indices vary significantly. Virulence and plant dry mass increasing are indices widely used in order to reveal well matched rhizobia strain and variety of legume host plant for practice demand. The aim of the research was to study how allelic polymorphism of symbiotically significant genes...

Annually, worldwide the food industry generates huge quantities of plastic packaging (polyethylene, polypropylene, polystyrene, polyethylene terephthalate, etc.) and due to their chemical structure takes hundreds of years to degrade, thus creating major environmental problems. Therefore, the aim of this study was to develop biodegradable films that can be used for food packaging and to identify their physical and mechanical properties. The biodegradable films were obtained using 150 ml Milli-Q water and different ...

The European Green Deal is one of the attempts to make our economy more sustainable in the long run, and these issues have been increasingly raised in Latvia over the recent years. Promoting changes in daily food consumption habits could also improve people’s quality of life in the future. In a globalized food system, small and local businesses are often being pushed out of the market, which is divided between the big producers and food chains. However, food is also part of each country’s unique cultural heritage ...

In the last years, the cultivation of methane-oxidizing organisms has been sort of great interest, because it allows to obtain value products using accessible raw materials, such as natural gas. The products obtained during the cultivation of mixed culture based on Methylococcus capsulatus can be used in agriculture, medicine and other fields. Nucleic acids and their derivatives are one of the most popular components that can be produced during the comprehensive processing of bacterial biomass. As a result of the ...

Zein proteins belong to the class of prolamin proteins which, in maize, represent more than 60% of the total endosperm protein. Based on their molecular weight, sequence and solubility, zeins are classified into four different zein structures (?, ?, ?, ?). The major maize seed storage proteins, zeins, are deficient in lysine and tryptophan content, which contribute to the poor nutritional quality of corn. Therefore, we report here on an original and novel process, which consists in successive grinding and sieving ...

An interest to bacteriocins as natural alternative for antibiotics and chemical preservatives is grow at present. The selection of novel strains producing antimicrobial peptides is in focus. The probiotics are preferable because they are recognized as safe and thereby can be applied in medications, foods, feeds. Moreover, the benefits of probiotics for human and animal health is proved. The growth kinetics of industrial probiotic strains Lactobacillus plantarum B-11007, Lactobacillus salivarius B-2214 ? Lactobacil...

PVP-I (povidone-iodine) or Betadine, molecular iodine combined with poly-(1-vinyl-2-pyrrolidone), has been reported as antimicrobial agent. It is also as a promising chemopreventive agent against cancer. In this study the antiproliferative effect of PVP-I on rhabdomyosarcoma (RD) and other cancer cell lines (AGS, HeLa, HepG2, and MiaPaCa2) was examined. Cancer cells were treated with PVP-I and assessed for cell proliferation, metabolic activity, colony forming assay, apoptosis, and the fluorometric assay of the ce...

The purpose of the work is to determine the patterns of influence of biotechnological and physical methods of processing raw materials on the quantitative indicators and amino acid composition of protein concentrates obtained from chickpea grain. With the use of enzyme preparations, the yield of soluble chickpea protein reached up to 60% of the total content in raw materials. The regularities of the effect of ultrasonic treatment on the solubility of the proteins of the suspension of chickpea flour with an increas...

In Siberia and the Far East (Asian part of Russia), regions with special climatic condi-tions, 99 species of plants are described that are pharmacopoeial and some of them are official, that is, plants that are raw materials for the production of medicines. However, not all medicinal pharmacopeia plants growing on the territory of Russia are protected, so the number of such species may soon be in jeopardy. The prospects of using medici-nal plants of the Siberian and Far Eastern regions by the example of Amur maacki...

The cryoscopic temperature (-1,3°C) in rainbow trout samples was determined. The calculation of the values of the individual temperature storage mode was carried out, ensuring that the product contains no more than 40% of ice water. The calculation of the amount of ice water was carried out using the calculation method proposed by D. Ryutov. A value of 40% is taken as the maximum permissible ice content in deep-chilled fish. According to research by N. A. Golovkin, this percentage of ice water provides a product t...

In this research work, we examined the types and nature of bonds that are formed upon immobilization of peroxidase on three types of carriers: SiO2, Al2O3, Fe3O4. To solve this problem, the method of FTIR spectroscopy was used. As a result of the analysis, many spectra were obtained at all stages of enzyme immobilization. Analyzing the spectra of pure carriers and the spectra obtained after modification of the carriers,it can be said that polystyrene sulfonic acid does not form any chemical bonds with the carriers...

Soil saprophytic nitrogen-fixing nodule bacteria (rhizobia) of the Sinorhizobium meliloti species are formed symbiosis with alfalfa and can also be a host of bacteriophages. Rhizobia have a symbiotic and saprophytic life phase?s cycle. Nodules formed by rhizobia on alfalfa roots are destroyed at the end of the growing season and bacteria surviving in nodule threads and did not transformed into bacteroids are released into the soil microbiome. The last is enriched in bacteriophages, which can significantly decrease...

Currently, CRISPR/Cas systems are actively studied bacterial systems, due to the possibilitpossibility to apply them for targeted editing of eukaryotic genomes. CRISPR/Cas systems are belonged to adaptive immun immune system of bacteria and archaea acting against invading mobile genetic elements (MGEs: bacteriophages and integrative conjugative elements ). Curre Currently CRISPR/Cas systems were detected in genomes of 75% of known archaea and 36 % of bacteria strains . However, there are still quite less knowledge...

Nodule bacteria (rhizobia) forming nitrogen-fixing symbiosis with legumes have a multicomponent genome represented by chromosome and a number of plasmids of different sizes. Genes related to symbiotic activity of Sinorhizobium meliloti are located predominantly on two megaplasmids SMa and SMb (1.35 and 1.68 Mb, correspondingly). Genomes at about 80% of native rhizobia nodulating alfalfa contained cryptic plasmids with sizes varied from 7 up to 450 kb. Functional role of this class of plasmids remained unclear up t...

The ability to colonize on plant roots is recognized as one of the most important characteristics of the beneficial fungi. To study these properties, we have developed and created a system for cultivating fungal microorganisms under sterile conditions on the surface of cellulose cylindrical tubes, into which a nutrient solution simulating plant exudate was constantly fed. This system simulates the development of microorganisms on the surface of the root system and allows to study the interaction of microorganisms ...

In this work, we propose technology for processing protein-containing products - beans into protein components for nutrition, also the use of waste after protein in the energy industry. The technology includes several stages: extraction, separation of fractions, precipitation, concentration. The optimal conditions for each stage of the technological process were selected (the ratio of the sample to the extracting substance, pH, water module, volume of precipitant, the volume of washing fractions, drying conditions...

As a result of joint research of employees of the All-Russian Research Institute of Reclaimed Lands and Tver State Technical University, aerobic-anaerobic solid-phase fermentation of a mixture of organic animal waste and hard-to-hydrolyze raw materials of plant origin was developed. Biotransformation of the fermented mixture takes place over several days, and the pasteurization effect is achieved as a result of short-term processing of the fermentation product with ultrasound intensity of 50-60 W/sq.cm. Liquid ani...

At the present stage of development of the biotechnological industry, the most promising objects are enzymes of the oxidoreductase class, which are capable of catalyzing redox processes. One of the most studied enzymes of this class is horseradish root peroxidase. Its immobilization on various carriers allows the biocatalyst to be reused in various technological processes. In recent years, magnetic nanoparticles have been increasingly used as effective carriers of enzymes.In this work, we study the oxidation of 2,...

The main purpose of this paper is to evaluate the impact of industry wastewater containing synthetic textile dyes on the structure of activated sludge. The aim of the research is to qualify direct impact of the textile dyes, dosed in one week into the experimental system of wastewater treatment plant, on constitution, structure and function of activated sludge. Activated sludge samples (5 mL) were taken from the aeration tank of experimental waste water treatment plant before, during and after measurement and the ...

Alzheimer?s disease, the most common age-related dementia worldwide, is characterized by two pathologies: deposition of ?-amyloid plaque and neurofibrillary tangles of hyperphosphorylated tau. To this date, several factors, such as oxidative stress, metal ions, or genetic factors are associated with Alzheimer`s diseases, but there are still some question marks regarding: (i) how the misfolding and aggregation of A? occurs and which is the aggregation mechanism; (ii) how the metal ions binds to A? and how they init...

Manure is characterized by a complex multicomponent composition and can be considered as a source of nutrients for microorganisms and plants. Moreover, manure has a high level of biogenicity. Therefore, this waste can be considered as the main resource for organic fertilizer production. Currently, there are many technological solutions for processing of renewable animal and poultry waste. Fast solid-phase fermentation of the organic feedstock is a most promising technology. Five technological solutions for the fer...

Currently, one of the priorities for sustainable development is human life getting better with functional foods. Non-dairy matrices are considered as promising alternative for production of foods and beverages with probiotic microbes. The development of these new products appears to be a hot area in which probiotic cereal beverages occupy a special place. The corn, buckwheat, oats, rye, wheat and barley flour was studied. Two-stage enzymatic hydrolysis of the water suspensions of the flour with amylases and protea...

The aim of the work was to optimize the process of obtaining multicomponent protein compositions with high biological value and higher functional properties than the original vegetable protein products. Was realized studies to obtain biocomposites on the base of pea protein-oat protein and pea protein-rice protein. Developed composites was enriched with all limited amino acids. For each of the essential amino acids, amino acid score was 100% and higher. Protein products used in this compositions are not in major a...

The results of extraction of pectin from beet pulp of sugar waste production have been presented. Production of pectin will allow you to avoid importing this product from abroad, as well as reduce the negative impact of sugar factories on the environment. The developed scheme is environmentally safe. For the production of jelly confectionery products of various assortment, pectin consumption ranges from 8 kg for citrus to 26 kg for beet pectin per 1 ton of finished products. In comparison with other gelling agents...

Symbiotic nitrogen fixation is the most environmentally friendly method for the accumulation of biologically bound nitrogen. Nitrogen fixation is carried out by bacteria, including from the class of ?-proteobacteria. Global climate changes lead to an increase of agricultural areas subjected to salinity. Current knowledge about the effect of high-salt stress on nitrogen-fixing symbiotic ?-proteobacteria has the appearance of a puzzle. The genetic mechanisms determining salt tolerance have been studied in the most d...

The antibiotic resistance is one of biosphere scale problem created by not only the medicine, but veterinary, food industry and animal husbandry. Bacteriocins are defined as ribosomally synthesized peptides with narrow or in some cases broad antimicrobial activity producing by various bacteria, especially against closely-related species. Among bacteriocin producers food-grade lactic acid bacteria (LAB) provide an increase interest due to their safety, preservative ability and potential probiotic properties. Unlike...

To date, there is an increase in the incidence of skin and subcutaneous tissue diseases, despite the variety of treatment methods. One of the promising class of biomaterials for the treatment of skin diseases can be coatings based on hydrogels. The purpose of this study was to obtain collagen-acrylic hydrogels with various technological parameters (varying the synthesis temperature and the degree of neutralization of acrylic acid) with the specified properties by radical polymerization. The chemical structure of t...