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Acidophilic microorganisms oxidizing ferrous iron and sulfur are widely used in hydrometallurgy to extract different metals including copper, zinc, nickel, cobalt, and uranium from sulfide ores. The activity of microorganisms and screening of microbial strains capable of biooxidation of ferrous iron and sulfur compounds under different conditions, i.e. in the present of different toxicants, which may be present in treated ores, are crucial for the development of biohydrometallurgical technologies. Different factor...

Oilseed rape (Brassica napus L.), a crop with great economic value, is grown for oil-rich seeds and after soybean is the second most common oil crop. According to literature, oilseed rape has a great demand for sulphur and boron, mainly for synthesis of glucosinolates and sulphur aminoacids. The present research was developed during agricultural year 2017-2018 in Ialomita County using Jumper rapeseed hybrid. The objective of the study was to evaluate the influence of mineral fertilization combined with boron and s...

The study presented in this paper is related to the extraction of humic lignite substances and the separation of fulvic acids in order to introduce them into a fertilizing matrix and to evaluate the effect on tomato culture. Fulvic acids have biostimulatory effect on agricultural crops and have significant uses in agriculture. For the characterization of fulvic acids (FA), the total acidity and content of carboxylic groups were determined. The separate fulvic acids were introduced into a matrix containing nutrient...

Plants have been considered as materials for alternative medicine and sources of pharmaceutical drugs. Despite the therapeutic advantages of medicinal plants, some of their constituents may be potentially toxic, mutagenic, carcinogenic, or teratogenic potentials. Therefore, the aim of our study is to determine the cytogenetic and genotoxic potentials of aqueous extract of Plantago lanceolata regarding quality, safety and efficiency. The cytogenetic effects were studied by the calculation of the mitotic index, by t...

Food processing wastes are the end products of the food processing industries which cannot be recycled or used for other purposes. These are the non-product flows of raw materials whose economic values are less than the cost of collection and recovery for reuse; and therefore discard as a waste. These wastes pose increasing disposal and potential severe pollution problems and represent a loss of valuable biomass and nutrients. Onion solid wastes (OSW) were used as raw material to produce polyphenol and extracts wi...

The molecular characterization of Genetic diversity of ten bread wheat (Triticum aestivum L.) varieties was evaluated by studying high molecular weight (HMW) glutenin subunits. Polymorphism of HMW-SG war carried on by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). Among genotypes analyzed, twelve polymorphic bands were identified at the three loci Glu-A1, Glu-B1 and Glu-D1. The analysis of HMW-GS reveals a great diversity at the level of the three loci and more particularly on the locus (Gl...

The search for new microorganisms ? sources of thermostable and thermoactive amylolytic enzymes is an important direction for the efficient processing of plant raw materials at high temperatures. A bacterial culture obtained from the thermal spring in the southeast of Iceland was used in the research. The identification of bacterial species by 16S rRNA gene sequencing has shown that the detected bacteria belong to the species Geobacillus kaustophilus. Microorganisms grown on a medium with the following composition...

Due to their huge volumes, toxicity and destruction resistance petroleum and petroleum products are the most important polluters. The source of oil pollution is not only oil extraction processes, but also oil transportation, refining and use. Ecologically safe and effective way to clean the environment off petroleum hydrocarbons is bioremediation. The effectiveness of oil-oxidizing biological products is ultimately determined by the activity of contained hydrocarbon oxidizing bacteria strains. The search, isolatio...

Technological parameters of the introduction of grapes into the in vitro culture have been improved. The use of growth regulators (6-BAP, 2iP, kinetin) contributed to the enhancement of rhizogenesis and an increase in the coefficient of in vitro plant reproduction. New methods for adapting grape plants that were in vitro improved to in vivo conditions at the reproduction stage have been studied. It has been shown that all concentrations of lingo-humate had a positive effect on plants, which in turn contributed to ...

The development of the most efficient technology is extremely important to synchronize work on the laying yielding strawberry parent plants. The main link here should be the material obtained in vitro. The most vulnerable element of in vitro technology is adaptation to non-sterile conditions, when it is very difficult to simultaneously control a large number of factors and when very large plant losses are possible. The fundamentally new method of adapting microplants to hydroponics has been tested and allows us to...

Amiodarone (AMD) is an antiarrhythmic agent included in the 2nd class according to the biopharmaceutical classification of drug substances. The oral bioavailability of AMD in conventional pharmaceutical formulations exhibits large interindividual variations due to the rapid dealkylation of the molecule to desethylaminodarone (inactive metabolite) and due to low hydrosolubility. The complexation of the drug substance in the central cavity of various kinds of cyclodextrins is one of the most effective methods for op...

The formation of spring wheat harvest in the forest-steppe of the Krasnoyarsk territory is influenced by highly varying biotic and abiotic factors that are often stressful. Many researchers associate the magnitude of crop variation in time and space with the nature of the development and variability of elements of the structure of the crop, which is largely determined by the biological characteristics of the variety, its plasticity. The purpose of research is to evaluate the varieties of spring wheat yield and env...

The aim of this study is to evaluate the influence of adulteration agents (glucose, fructose, inverted sugar, and hydrolysed inulin syrup and malt wort) addition into honey on texture parameters (viscosity, hardness, adhesion, springiness, cohesiveness, chewiness and gumminess). For this purpose, 5 honey samples of different botanical origins (acacia, honeydew, polyfloral, sunflower and tilia) has been adulterated with different percentages 0, 5%, 10%, 20%, 30%, 40% and 50% respectively with glucose, fructose, inv...

Lens culinaris (lentil) has high concentrations of phenolic acids, polyphenols, proteins, dietary fibers and vitamins. Lentil microgreen has been shown to possess antidiabetic activity, anti-inflammatory, anti-cancer activity and antioxidant activity. In the present paper, we have studied the influence of brewer?s spent yeast, typical breweries? waste product, in the germination and growth substrate on the lentil sprouts. The changes in the concentration of total polyphenols and antioxidant activity were monitored...

Water deficit is the major environment factor affecting bread wheat (Triticum aestivum L.) grain quality under Mediterranean climate. Many ecological regions in Algeria are suitable for the production of bread wheat, and therefore the technological quality of this wheat should be improved. The determination of the technological quality of bread wheat (Triticum aestivum L.) requires a strong investment of time and money on behalf of the flour industry. For this reason, a field experiment was carried out over a 1-ye...

The sustainable agriculture requires a series of complex, individual but interrelated and dependent on each other processes that need to be efficiently organized in stages to ensure good overall results. Therefore, the success of a given product is based on a triangle formed by the farmer, the technology and the concept of services and counseling. Services and counseling are based on collected data. Data generated from modern agricultural activities is extracted with the usage of various sensors embedded in agricu...

A studies were conducted of the template method for the formation of a 3D scaffold. As templates, previously prepared particles of NaCl, lactose, and calcium alginate were investigated. The particle-shaped template was introduced in different volume ratios with a PLGA solution containing dispersed agglomerates of hydroxyapatite nanoparticles (HAP NPs). For functionalization of the surface, scaffolds were immersed in an aqueous solution of chitosan for the purpose of physical adsorption of chitosan molecules on the...

When fermenting dairy environments under conditions other than optimum, due to the formation of an exopolysaccharide capsule in lactic acid bacteria, resistance to aggressive external factors, such as acidity, temperature, and ultrasound, increases. The synthesis of exopolysaccharides contributes to the intensification of milk fermentation and an increase in the proportion of physically and chemically bound moisture. The purpose of the experiment was to study the activity of polysaccharide biosynthesis by a consor...

The hawthorn (Crataegus monogyna) is very widespread in Romania. In the southwestern part of the Banat, on the hilly and mountainous area, the hawthorn has very representative population. It is very often found in those areas in small groups of 4-15 shrubs and is most common found in the selvage of the forests and on the areas where are old forest roads, along the gardens of the meadows of the rivers from mountain areas. Also, hawthorn plants are often used by local population from these areas to make green fences...

Ketoprofen is well-known drug due to anti-inflammatory, analgesic and antipyretic properties, and is widely used in the treatment of various acute and chronic inflammatory diseases, including rheumatoid arthritis, osteoarthritis, ankylosing spondylitis as well as menstrual abdominal cramps. Its therapeutic benefits in the prevention of various cancers including colorectal and lung cancers as well as in the treatment of neurodegenerative disorders such as Alzheimer?s disease, Parkinson?s disease have been establish...

The biosynthesis of noble metal nanoparticles using microorganisms (?green synthesis of nanoparticles?) has recently become widespread for medical applications. The characteristics of the resulting nanoparticles depend on the microorganisms used and the process conditions. Screening of a number of methylotrophic bacterial communities of a practical biotechnological value was carried out to determine those ones, which were able to synthesize silver and palladium nanoparticles at high rate and efficiency. In several...

The paper emphasize the correlation among the metals content in different samples of some individual macrophyte algae species with different contribution, as part of residual algae biomass along Romanian Black Sea Coast, that could be used as a potential new biostimulator-regenerator recommended for improving the quality of the vineyard soils from Dobrudja region, Romania. Concentrations and sources of copper, zinc, iron, chromium, manganese, cadmium, lead and aluminium were analyzed. For the metals content determ...

Organs-on-chip allow for establishment of physiologically relevant models of human tissues and organs, and specifically of blood-brain barrier (BBB) and neurovascular unit (NVU) in their normal or pathological state. BBB/NVU integrity and functions are compromised in course of different diseases or injuries, like traumatic brain injury, stroke, multiple sclerosis, arteriovenous malformations, vascular dementia, Alzheimer?s disease, atherosclerosis, brain cancer, inflammation, genetic disorders, and many more. In t...

Synbiotics are defined as a composition of probiotics and prebiotics with synergistic reinforcing effects. In practice, only growth stimulation is often took into account when justifying the effectiveness of a synbiotic composition. However, the effect of prebiotics to the interaction of probiotic and pathogenic bacteria during co-culture fermentation was examined in a range of researches. The influence of commercial prebiotics on the ability of probiotics (bifidobacteria and lactobacilli) to suppress the growth o...

This study aimed to identify a rational treatment for soil and determine the efficiency of the use of chelated liquid microfertilizers on the yield of spring hard wheat crops in chernozemic soils of the Samara Region of Russia. The study was conducted as a two-factor field experiment using a recognized variety of spring hard wheat:?Bezenchukskaya stepnaya?. The soil of the experimental field was typical medium-deep heavy loamy chernozem. Data on some agrophysical properties of the soil, weed infestation of crops, ...

The aim of the work was to optimize the process of obtaining multicomponent protein compositions with high biological value and higher functional properties than the original vegetable protein products. Initially, studies were carried out to obtain a biocomposite composition of dry wheat gluten (DWG) - pea protein, DWG-potato protein, potato protein-oat protein. Composites are enriched with all three limiting amino acids: lysine, threonine, sulfur-containing amino acids. For each of the essential amino acids, amin...

Poultry meat and table eggs are known to be a popular food items all over the world. The nutritional value of poultry white meat is of great value over the red meat. It contains considerable amount of protein, minerals, vitamins, and fat. In order to ensure a good quality of poultry products, the flock should be a good health and free from infectious agents. Antibiotics were widely used to prevent diseases in poultry flocks. However, using antibiotics for long time may lead to microbes that are resistant to drugs....

Morphogenesis of structural elements of secondary xylem is associated with the biosynthesis of a massive secondary cell wall consisting of three layers, differing in mass, composition and structural organization. Cellulosic microfibrils are basic structural elements fixing cell walls. The participation of cellulases in cellulose biosynthesis is shown. Overexpression of cellulases (EGase) increased cell expansion and elongation and resulted in an increase in plant growth and biomass production. However, the precise...

The purpose of the study was the laboratory testing of the pendant seeder-spreader (production of China) as part of the universal unmanned aircraft system "OSA HEXA" based on UAV "OSA" (developer and producer was Bozon LLC, Moscow), in connection with which we solved the following tasks: 1) determination and assessment of performance indicators of the functioning of spreading device; 2) selection of the best modes of application of the test object; 3) selection of significant factors affecting the quality indicato...

In this study we performed a comparative analysis of the flavonoid compounds found in the composition of some species belonging to the Cucurbitaceae family, namely: cucumber (Cucumis sativus), bitter melor (Momordica charantia), zucchini or courgette (Cucurbita pepo) and horned melon (Cucumis metuliferus). The flavonoids in these species were identified by high performance liquid chromatography. The Cucumis metuliferus plant extract had the highest content in phenolic compounds (14.424 mg/L), while the Cucumis sat...

Consumption of muffins has become very popular around the world. Various ingredients have been used to obtain these muffins and different recipes can be found in the literature. In this study three different samples (S) of muffins (S1- with wheat flour, S2 ? with wheat flour: almond flour (1:1) and S3 ? with almond flour) were prepared and investigated. Muffins also contain, besides flour: eggs, coconut oil, sugar, nuts, cherries and orange juice in the same proportions for all samples. The composition is dosed in...

most digestible form of it (puree), the potato was one of the first vegetable foods to be given to children after weaning and to convalescents who resumed their normal diet. Potato is part of the group of tubers vegetables, having as an edible organ the tubercles, which are thickened underground parts that accumulate reserve nutrients, especially starch. Potato?s food value is higher than that of other root vegetable, due to starch, vitamins, sugars and minerals salts. Taking into account the way of use, there are...

Model for a change of the biosystems states on the grounds of Markov chains is offered in the work. One of the main problems of management of the biosystems states is the study of the mechanisms, which determine dynamic features of the processes, taking place in the natural environment. Therefore, there is a necessity in the development of special methods, allowing to study dynamic properties of natural objects without subjecting them to ruination. Naturally, a mathematical modeling method may satisfy this demand ...

New functional foods produced by fermentation of pretreated with amylolytic and proteolytic enzymes cereal raw materials suspensions by probiotics are presented. Whole meal wheat flour comprising organic compounds and microelements was used as source of nutrients. Duozyme (Novozymes) amylases (1% by weight of the substrate) and Protex 40E (Genencor) proteases (2% of the protein content in flour) were applied for hydrolysis. The starter culture was one of the gram-positive probiotic lactic acid bacteria Lactobacill...

The increasing amount of fiber on wheat flour brings some deleterious effects on dough structure, such as the increasing of dough stickiness and fermentation tolerance decreasing. Also it has a negative influence against other properties of dough such as handling and rising properties. Fiber addition usually causes a decrease in bread volume and breadcrumb loses its elasticity. So, we had investigated if the xylanase addition could counteract those deleterious effects. Positive action of xylanase in dough could be...

Frozen fish is the primary raw material used in the industrial preparation of semi-finished ready to eat fish-based products. Ready to eat fish product manufacturers receive frozen fish raw materials from suppliers and defrost it before processing. Such raw materials are commodities; frozen fish are easily transported around the world, can be stored for a long time, and used for further processing as required, evenly distributing the company?s production capacity throughout the year and, thus, optimising costs. No...

Investigated were edaphic conditions of post-technogenic landscapes of the Yavoriv sulfur quarry (YSQ). Mycological characteristics of the devastated lands have been identified. The peculiarities of natural regeneration of the vegetation cover on the devastated lands of YSQ (the species, coenotic and ecological structures, directions of transformation of self-regenerating phytocoenoses) have been revealed. Analyzed are peculiarities of sulfur transformation by microscopic fungi. Investigated was the role of mycorr...

The purpose of the research was to study the regenerating potential of tomato callus culture depending on the hormonal composition of the medium, genotype, and age of the explant. Introduction in vitro culture was performed according to the standard method. As a basis, the mediums were served -Murashige and Skoog (MS) and Linsmaier and Skoog (LS). Growth hormones were added in various combinations and concentrations. As initial material, samples of local and foreign selection tomato varieties. As explants, cotyled...

This paper presents the stages of embryonic and postembryonic development in Polyodon spathula (Polyodontidae) under the conditions of the acclimatization area at Fish Culture Research and Development Station Nucet, Dambovita county, Romania. Embryonic development at the paddlefish is carried out in the temperature range of 14-18o C with an optimum of 16o C. For the induction of maturation and ovulation, the Nerestin 5A hormone was used. The size of the eggs is between 2.7-3 mm and light gray to gray-black in colo...

A vital role in detecting eye disorders is played by the analysis of the human retina using medical imaging methods. The main objective of this study is to improve the detection of retinal blood vessels by ameliorating the overall quality of non-uniform illumination conditions, removing image artifacts, connecting the broken vessels, and generating accurate segmented retinal vascular systems. A three-stage edge detection framework is proposed. First, image denoising, contrast enhancement, stretched decorrelation a...

Shoot cultures of the essential oil bearing plant Artemisia alba Turra (Asteraceae) were studied with the aim to elaborate the effect of in vitro rooting on cytokinin (CK) conjugation and chloroplast architecture in vitro. For this purpose, two in vitro morphotypes were induced ? (1) the root developing morphotype on plant growth regulator-free as well as on auxin indole-3-butyric acid (IBA)-containing media and (2) the root suppressed morphotype, obtained by a combination of IBA with CK N6-benzyladenine (BA). End...

Creation of food products with a predictable biopotential and consumer properties is an important and promising direction for the development of the food industry in the world. In this regard, the wheat germ cake is not used efficiently, which is mainly due to the low stability of their quality during storage. The initiator of the oxidative processes of the cake is a highly active lipase. We proposed the use of fumaric acid as an antioxidant and conducted research to study the effect of this inhibitor on the oxida...

Neurodegenerative diseases such as Alzheimer's and Parkinson's affect an increasing number of people. Their etiology is related to the formation of fibrils and protein aggregates. Therefore, new therapeutic strategies and compounds are required to prevent aggregation of ?-amyloid (A?) peptides. There are some neuropeptides that play an important role in the immune regulation, as well as in neuroprotection; therefore, they might be possible candidates for future drug development. Consequently, our experiments were ...

As a basic number, a genealogical line of Holstein-Friesian cows within SCDA Craiova was selected with common genes from the bull STARBUCK (Canada) brought by their grandchildren 51801 and 52150 via BESNE BUCK (France), 52506 and 52507 via SABBIONA BOOKIE USA) and the great grandchild 52569 via FATAL (USA); Genetic assays for production are based on 305 days of lactation production in the Maturity Equivalent (ME) of each cow. The dairy cows within SCDA Simnic are subject to official control of milk production. Thi...

The analysis of reproductive performance in selected cows within SCDA Simnic Craiova, the obtention of the first generation and the exploration of the biological characteristics of the obtained youth, took into account the following breeding indices: calving interval (CI), determined as days between two consecutive parturitions; (SP) service period, determined as days from parturition to conception; number of inseminations per conception (NIC); Conception rate (RCv) determined as a percentage of fertilized insemin...

The paper is part of an important Scientific Research Project, VALINTEGR 2018-2020, in which some food supplements from the Cannabis Sativa and Helianthus tuberosus residues will develop. This study highlights the influence of some processing processes on the activity of some oxidoreductases from Canabis Sativa. The hemp seeds contain the daily needs of essential fatty acids: omega 6 and omega 3, which keep the clean of blood vessels and prevent cardiovascular disease. Also, hemp seeds contain 40% fat, 30% protein...

Industrially defatted non-dehulled sunflower meal (SFM) with a protein content 22%, fibre 38% and cellulose 23% was separated into several fractions: protein fraction and lignocellulosic fraction, then they can be used for bioconversion into value added products. The protein concentrate (PC) contained 83% of protein. Hydrolysis of this concentrate by the commercial proteases, such as Proteinase T, Protex 7L, Protex 40E (Danisco Genecor, Denmark) and Pancreatin (Biosynthesis, the Russian Federation) can transform t...

It was studied susceptibility of starch granules of different types to hydrolysis by enzymes both in the gelatinized and native state. Starch was evaluated by chemical composition, morphology, physical and chemical properties and compared with respect to the action of amylolytic enzymes traditionally used to produce sugary starch products (alpha-amylase, glucoamylase). Enzymatic susceptibility of the samples was evaluated by accumulation of reducing matters during the hydrolysis of 1% starch suspension in gelatini...

Lake Onego is the second largest lake in Europe and it is situated in the Northwest of Russia. Benthal deposits of lake Onego are known to contain a large number of iron ore, and that affects the bacterial communities present in the lake. Some bacteria have a defense mechanism against the toxicity of metal ions which involves formation of metal nanoparticles. Bacterial synthesis of metal nanoparticles has a wide range of applications in industry due to its low cost, non-toxicity and eco-friendliness. Due to their ...

Nutrition is the most important environmental factor that influences the body (Pavlov). About food is said to be a "necessity that gives birth to all the others" or "the pleasure of all ages, of all conditions, of all countries and all the days, which can be associated with all the pleasures and we remain at the last to console ourselves from the loss of others " It also confirms that: "you do not live from what you eat, but from what you digest." By the name of fresh meat products, we mean the meat products made ...