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An interest to bacteriocins as natural alternative for antibiotics and chemical preservatives is grow at present. The selection of novel strains producing antimicrobial peptides is in focus. The probiotics are preferable because they are recognized as safe and thereby can be applied in medications, foods, feeds. Moreover, the benefits of probiotics for human and animal health is proved. The growth kinetics of industrial probiotic strains Lactobacillus plantarum B-11007, Lactobacillus salivarius B-2214 ? Lactobacil...

Honey is the unique product which forms in fermentation of flower nectar by bees. It has been known for its healing properties for many centuries. The prevail component of the honey is monosaccharides (first of all glucose and fructose). Nevertheless, di-, tri- and oligosaccharides as well as proteins and pigmenting phenolic compounds can be detected. The specific saccharides of honey are in interest as prebiotics. The phenolic compounds can suppress different microbes including pathogens and food contaminants. Th...

Summary. Aluminum, entering the human body, has the property of accumulation, modulates immunoregulation and metabolism, especially mineral. It also exhibits the properties of a sensitizer and neurotoxicant, mutagen and carcinogen. And in the case of aerogenic exposure, the target object is additionally the upper and lower respiratory tract system. Exposed by aluminium children are especially inclined to occurrence of negative effects. The research is aimed to study the immune status of children of primary school ...

PVP-I (povidone-iodine) or Betadine, molecular iodine combined with poly-(1-vinyl-2-pyrrolidone), has been reported as antimicrobial agent. It is also as a promising chemopreventive agent against cancer. In this study the antiproliferative effect of PVP-I on rhabdomyosarcoma (RD) and other cancer cell lines (AGS, HeLa, HepG2, and MiaPaCa2) was examined. Cancer cells were treated with PVP-I and assessed for cell proliferation, metabolic activity, colony forming assay, apoptosis, and the fluorometric assay of the ce...

The purpose of the research is to develop multifunctional biological products based on the utilization of organic waste and raw materials from components of animal and plant origin; to study their impact on the restoration of soil fertility and productivity of field and garden crops. As a result, the assortment line of biological products of the Agrotonic trademark Was developed: "flower Grower", "Gardener", "vegetable Grower", "field Grower" and "forest Grower". Each type of "AG-rotonika" is also divided into sub...

The purpose of the work is to determine the patterns of influence of biotechnological and physical methods of processing raw materials on the quantitative indicators and amino acid composition of protein concentrates obtained from chickpea grain. With the use of enzyme preparations, the yield of soluble chickpea protein reached up to 60% of the total content in raw materials. The regularities of the effect of ultrasonic treatment on the solubility of the proteins of the suspension of chickpea flour with an increas...

In 2018 the squid catch in the Russian Federation amounted to about 102 thousand tons. Different kinds of products are made from squid (dried, air-dried and smoked squid, preserves, canned food, culinary products), but frozen squid makes a great part of them. In the production of squid carcasses, about 52.3% of waste is generated, of which 23.2% accounts for the liver and 76.8% for the entrails, tentacles and head. Therefore, about 54 thousand tons of waste is generated annually, where 41 thousand tons accounts fo...

In Siberia and the Far East (Asian part of Russia), regions with special climatic condi-tions, 99 species of plants are described that are pharmacopoeial and some of them are official, that is, plants that are raw materials for the production of medicines. However, not all medicinal pharmacopeia plants growing on the territory of Russia are protected, so the number of such species may soon be in jeopardy. The prospects of using medici-nal plants of the Siberian and Far Eastern regions by the example of Amur maacki...

The cryoscopic temperature (-1,3°C) in rainbow trout samples was determined. The calculation of the values of the individual temperature storage mode was carried out, ensuring that the product contains no more than 40% of ice water. The calculation of the amount of ice water was carried out using the calculation method proposed by D. Ryutov. A value of 40% is taken as the maximum permissible ice content in deep-chilled fish. According to research by N. A. Golovkin, this percentage of ice water provides a product t...

In this research work, we examined the types and nature of bonds that are formed upon immobilization of peroxidase on three types of carriers: SiO2, Al2O3, Fe3O4. To solve this problem, the method of FTIR spectroscopy was used. As a result of the analysis, many spectra were obtained at all stages of enzyme immobilization. Analyzing the spectra of pure carriers and the spectra obtained after modification of the carriers,it can be said that polystyrene sulfonic acid does not form any chemical bonds with the carriers...

Soil saprophytic nitrogen-fixing nodule bacteria (rhizobia) of the Sinorhizobium meliloti species are formed symbiosis with alfalfa and can also be a host of bacteriophages. Rhizobia have a symbiotic and saprophytic life phase?s cycle. Nodules formed by rhizobia on alfalfa roots are destroyed at the end of the growing season and bacteria surviving in nodule threads and did not transformed into bacteroids are released into the soil microbiome. The last is enriched in bacteriophages, which can significantly decrease...

Introduction Violations of autonomic nervous regulation are a multifactorial pathology, while environmental features are an important risk factor for this pathology. Excessive exposure to neurotropic technogenic chemical factors, including benzene, to the biological environment of the human body induces malfunctions of adaptive-compensatory mechanisms and forms an increased risk of developing disorders of autonomic regulation associated with the immune system, which are realized against the background of polymorph...

Drinking water contamination with technogenic haptens forms an imbalance in the adaptive capabilities of the immune system in children. The aim of this work is to study the features of laboratory markers of the cytokine profile in children, probabilistically associated with an increased content of chloroform in the blood. Materials and methods. The study involved 283 children living in conditions of chronic low-level oral exposure to hyperchlorination products of drinking water, the control for which was a conting...

Currently, CRISPR/Cas systems are actively studied bacterial systems, due to the possibilitpossibility to apply them for targeted editing of eukaryotic genomes. CRISPR/Cas systems are belonged to adaptive immun immune system of bacteria and archaea acting against invading mobile genetic elements (MGEs: bacteriophages and integrative conjugative elements ). Curre Currently CRISPR/Cas systems were detected in genomes of 75% of known archaea and 36 % of bacteria strains . However, there are still quite less knowledge...

Nodule bacteria (rhizobia) forming nitrogen-fixing symbiosis with legumes have a multicomponent genome represented by chromosome and a number of plasmids of different sizes. Genes related to symbiotic activity of Sinorhizobium meliloti are located predominantly on two megaplasmids SMa and SMb (1.35 and 1.68 Mb, correspondingly). Genomes at about 80% of native rhizobia nodulating alfalfa contained cryptic plasmids with sizes varied from 7 up to 450 kb. Functional role of this class of plasmids remained unclear up t...

The ability to colonize on plant roots is recognized as one of the most important characteristics of the beneficial fungi. To study these properties, we have developed and created a system for cultivating fungal microorganisms under sterile conditions on the surface of cellulose cylindrical tubes, into which a nutrient solution simulating plant exudate was constantly fed. This system simulates the development of microorganisms on the surface of the root system and allows to study the interaction of microorganisms ...

In this work, we propose technology for processing protein-containing products - beans into protein components for nutrition, also the use of waste after protein in the energy industry. The technology includes several stages: extraction, separation of fractions, precipitation, concentration. The optimal conditions for each stage of the technological process were selected (the ratio of the sample to the extracting substance, pH, water module, volume of precipitant, the volume of washing fractions, drying conditions...

As a result of joint research of employees of the All-Russian Research Institute of Reclaimed Lands and Tver State Technical University, aerobic-anaerobic solid-phase fermentation of a mixture of organic animal waste and hard-to-hydrolyze raw materials of plant origin was developed. Biotransformation of the fermented mixture takes place over several days, and the pasteurization effect is achieved as a result of short-term processing of the fermentation product with ultrasound intensity of 50-60 W/sq.cm. Liquid ani...

At the present stage of development of the biotechnological industry, the most promising objects are enzymes of the oxidoreductase class, which are capable of catalyzing redox processes. One of the most studied enzymes of this class is horseradish root peroxidase. Its immobilization on various carriers allows the biocatalyst to be reused in various technological processes. In recent years, magnetic nanoparticles have been increasingly used as effective carriers of enzymes.In this work, we study the oxidation of 2,...

The main purpose of this paper is to evaluate the impact of industry wastewater containing synthetic textile dyes on the structure of activated sludge. The aim of the research is to qualify direct impact of the textile dyes, dosed in one week into the experimental system of wastewater treatment plant, on constitution, structure and function of activated sludge. Activated sludge samples (5 mL) were taken from the aeration tank of experimental waste water treatment plant before, during and after measurement and the ...

Alzheimer?s disease, the most common age-related dementia worldwide, is characterized by two pathologies: deposition of ?-amyloid plaque and neurofibrillary tangles of hyperphosphorylated tau. To this date, several factors, such as oxidative stress, metal ions, or genetic factors are associated with Alzheimer`s diseases, but there are still some question marks regarding: (i) how the misfolding and aggregation of A? occurs and which is the aggregation mechanism; (ii) how the metal ions binds to A? and how they init...

Manure is characterized by a complex multicomponent composition and can be considered as a source of nutrients for microorganisms and plants. Moreover, manure has a high level of biogenicity. Therefore, this waste can be considered as the main resource for organic fertilizer production. Currently, there are many technological solutions for processing of renewable animal and poultry waste. Fast solid-phase fermentation of the organic feedstock is a most promising technology. Five technological solutions for the fer...

Currently, one of the priorities for sustainable development is human life getting better with functional foods. Non-dairy matrices are considered as promising alternative for production of foods and beverages with probiotic microbes. The development of these new products appears to be a hot area in which probiotic cereal beverages occupy a special place. The corn, buckwheat, oats, rye, wheat and barley flour was studied. Two-stage enzymatic hydrolysis of the water suspensions of the flour with amylases and protea...

The aim of the work was to optimize the process of obtaining multicomponent protein compositions with high biological value and higher functional properties than the original vegetable protein products. Was realized studies to obtain biocomposites on the base of pea protein-oat protein and pea protein-rice protein. Developed composites was enriched with all limited amino acids. For each of the essential amino acids, amino acid score was 100% and higher. Protein products used in this compositions are not in major a...

The results of extraction of pectin from beet pulp of sugar waste production have been presented. Production of pectin will allow you to avoid importing this product from abroad, as well as reduce the negative impact of sugar factories on the environment. The developed scheme is environmentally safe. For the production of jelly confectionery products of various assortment, pectin consumption ranges from 8 kg for citrus to 26 kg for beet pectin per 1 ton of finished products. In comparison with other gelling agents...

Symbiotic nitrogen fixation is the most environmentally friendly method for the accumulation of biologically bound nitrogen. Nitrogen fixation is carried out by bacteria, including from the class of ?-proteobacteria. Global climate changes lead to an increase of agricultural areas subjected to salinity. Current knowledge about the effect of high-salt stress on nitrogen-fixing symbiotic ?-proteobacteria has the appearance of a puzzle. The genetic mechanisms determining salt tolerance have been studied in the most d...

Modern industrial biotechnology considers extremophilic microorganisms and, in particular, extreme halophiles as promising producers for the bioeconomy of the future. Extremely halophilic bacteria and archaea can be used for the production of carotenoids, polyhydroxyalkanoates, polysaccharides, osmoprotectors, thermostable enzymes, photosystem proteins, and can also be sources of unique biologically active substances for medical purposes. The implementation of large-scale industrial processes with minimal requirem...

The main strategy of the world fishery complex is the development and implementation of technologies for deep processing of aquatic biological resources (WBR), including those that allow addressing the issue of filleting waste disposal. Using the existing technologies of processing, we get the amount of filleting waste of up to 80% or ~1.6 million tons of the average annual catch weight in the Russian Federation alone. However, it is known that fish skin and cartilage tissue are highly valuable technological raw m...

The antibiotic resistance is one of biosphere scale problem created by not only the medicine, but veterinary, food industry and animal husbandry. Bacteriocins are defined as ribosomally synthesized peptides with narrow or in some cases broad antimicrobial activity producing by various bacteria, especially against closely-related species. Among bacteriocin producers food-grade lactic acid bacteria (LAB) provide an increase interest due to their safety, preservative ability and potential probiotic properties. Unlike...

Currently, systems for encapsulating drugs are widely used to obtain drugs, since they provide both the preservation of the activity of the administered drug and the reduction of side effects from its administration on the gastrointestinal tract. Complexes of natural polysaccharides such as chitosan alginate and chitosan pectin are widely used as such carriers. These complexes have high biological resistance and biodegradability. In addition, they provide the release of the drug at neutral pH values, which corresp...

To date, there is an increase in the incidence of skin and subcutaneous tissue diseases, despite the variety of treatment methods. One of the promising class of biomaterials for the treatment of skin diseases can be coatings based on hydrogels. The purpose of this study was to obtain collagen-acrylic hydrogels with various technological parameters (varying the synthesis temperature and the degree of neutralization of acrylic acid) with the specified properties by radical polymerization. The chemical structure of t...

This study is devoted to studying the properties of a new polymer sorbent based on polyvinyl alcohol and carboxymethylcellulose using infrared spectroscopy and thermogravimetric analysis. The influence of calcium chloride on the performance characteristics of the sorbent, such as oil capacity, floatation, moisture content and polymer degradation, is studied. The cross-linked matrix has improved properties: increasing the oil capacity and flotation time, retention of the structure when the sorbent is on the water s...

The problem of obtaining compositions, that would act as biomaterials and fit all demands, is well-known. One the branch of application materials such is the hydrogels. High retention capacity of water and physiological fluids, sufficient physio-mechanical properties and safety of usage are ones of the most relevant specifications for medical hydrogels. It is quite hard to develop such formulations. Perspective initial substances for synthesis are gelatin (peptide native to human collagen) and tannin (natural comp...

Today, the analysis of the unique contribution to the formation of the ?genetic portrait? of regional features, including technogenic ones, which determines the occurrence of diseases and harm to health, is relevant. The use of modern critical genetic and cellular biotechnologies with the study of genetic polymorphism and thin cell subpopulations will allow us to catch the earliest violations of homeostatic indicators in geochemical technogenic provinces. The aim of the work is to assess the features of genetic in...

The study object is the research of the suitability of some maize hybrids (Zea mays L.) from Croatia. There were six cultivars of maize from Croatia: OSSK 403, OS 430, OSSK 515, OSSK 552, OSSK 617, OSSK 635 while as standard check was used hybrid NS 640 which is among the most used hybrids in Kosovo. Most of evaluated maize hybrids showed very high level of adaptability in agro-climatic condition of Kosovo. Six maize hybrids F1 generation from Croatia were tested, during the growing season, in agroclimatic conditi...

Fabrication of nanofibers is a field of research attracting a lot of interest from technologici scientists because of its great applications in many fields. In order to design the polymer drug delivery system, we had to select a polymer that is biocompatible, biodegradable, non-toxic and approved for medical use. One of the polymers that fulfill those requirements is poly (vinyl alcohol) (PVA). There have been various reports on the fabrication of PVA nanofibers with solvents as water, or water with different salt...

Within Europe 2020 Strategy the maxi Framework Programme Horizon 2020 cover a prominent role. SME Instrument is an implementation tool which had largely contributed to the development of innovative initiatives within the Small Medium Enterprises? system focusing on smart, sustainable and inclusive growth. SME instrument is staggered in three phases and the first two phases are the concrete vehicle to obtaining financing. This paper provides the concrete robustness of the accessions to 2019, i.e. the total number o...

The aim of the research is to develop multicomponent organic fertilizers based on nanostructured diatomite using organic waste and raw materials processing products, to study the indicators of agrophytocenoses and field crop yields. The paper uses the results of research on the development of multicomponent organic fertilizers, as well as their effectiveness in the experimental field of the Samara state agrarian UNIVERSITY in 2017-2019. (southern forest-steppe of the Volga region). One of the types of multicompone...